AMP-activated protein kinase regulates the function of tight junction.
| Project/Area Number |
26840068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
Tomoki Yano 大阪大学, 医学(系)研究科(研究院), 助教 (20546121)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞間接着 / タイトジャンクション / 細胞骨格 / AMP活性化プロテインキナーゼ / アドヘレンスジャンクション / AMP活性プロテインキナーゼ |
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| Outline of Final Research Achievements |
I found that the AMP-activated protein kinase (AMPK) phosphorylates several proteins of tight junction (TJ). I showed that when cingulin, it is tight junctional protein, was phosphorylated by AMPK, the molecular configurational change occurred. By exploiting this conformational alteration regulated by AMPK, I try to design a module that undergoes efficient fluorescence resonance energy transfer (FRET). FRET probe is possible to be a powerful tool to visualize the intracellular nutritional condition by observing the activity of AMPK.
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Report
(3 results)
Research Products
(2 results)
