The roles of planar cell polarity in non-epithelial tissues

• Project/Area Number: 26840075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Osaka University (2015-2016); Chiba University (2014)
• Principal Investigator: HASHIMOTO Masakazu 大阪大学, 生命機能研究科, 助教 (60580496)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 平面内細胞極性 / 軟骨細胞 / 鼻 / 収斂伸長 / ゲノム編集 / 顔面形成

Outline of Final Research Achievements

We show that convergent extension- like cellular behavior in the nasal septum cartilage elongate the nasal process in proximodistal axis during the development of mice. While Wnt5a, a typical non- canonical Wnt ligand, is expressed in the distal tip of the nasal process, Prickle1, one of the planar cell polarity (PCP) core factor is expressed in the nasal cartilage. Mutant mice of these genes have short and wide snout as well as the deviated nasal septum due to the lack of the polarity of the chondrocyte of the nasal septum. Furthermore, the induced ubiquitous expression of Wnt5a also resulted in the nasal hypoplasia resulting from the chondrocyte polarity and orientation defects. These results suggest that the nasal cartilage senses the Wnt5a gradient along the proximodistal axis, and polarize the chondrocytes by a Prickle1-dependent manner for the convergent extension movement to form the long and thin nose in mammals.

Research Products

• [Journal Article] Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. (2016)
  • Author(s): Hashimoto M, Yamashita Y, Takemoto T.
  • Journal Title: Developmental Biology Volume: 418 Issue: 1 Pages: 1-9
  • DOI: 10.1016/j.ydbio.2016.07.017
  • Peer Reviewed
• [Journal Article] Somatic cell reprogramming-free generation of genetically modified pigs. (2016)
  • Author(s): Tanihara F, Takemoto T, Kitagawa E, Rao S, Do LT, Onishi A, Yamashita Y, Kosugi C, Suzuki H, Sembon S, Suzuki S, Nakai M, Hashimoto M, Yasue A, Matsuhisa M, Noji S, Fujimura T, Fuchimoto D, Otoi T.
  • Journal Title: Sci Adv Volume: 14 Issue: 9
  • DOI: 10.1126/sciadv.1600803
  • NAID: 120006240045
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. (2015)
  • Author(s): Hashimoto M. and Takemoto T.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11315-11315
  • DOI: 10.1038/srep11315
  • NAID: 120006875680
  • Peer Reviewed / Open Access