Functional analysis of shootin1 during zebrafish tissue morphogenesis

• Project/Area Number: 26840081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Urasaki Akihiro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40550083)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: shootin1 / ゼブラフィッシュ / 組織形成

Outline of Final Research Achievements

In addition to shootin1, we found two novel shootin family members (shootin 2 and shootin 3). All three shootin genes were expressed in zebrafish embryos. In particular, shootin 1 and shootin 3 were expressed maternally and were expressed in the lateral line primordia in developing zebrafish embryos. Mutant analysis suggests that shootin 3 is involved in the early embryonic development and that shootin 1 and shootin 3 are involved in the formation of lateral lines, which are organs that sense water flow.

Research Products

• [Journal Article] Identification of a shootin1 isoform expressed in peripheral tissues (2016)
  • Author(s): Yasuna Higashiguchi, Kazuhiro Katsuta, Takunori Minegishi, Shigenobu Yonemura, Akihiro Urasaki, Naoyuki Inagaki
  • Journal Title: Cell Tissue Res. Volume: 366 Issue: 1 Pages: 75-87
  • DOI: 10.1007/s00441-016-2415-9
  • NAID: 120005818453
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sox7 controls arterial specification in conjunction with hey2 and efnb2 function (2015)
  • Author(s): Hermkens DM, van Impel A, Urasaki A, Bussmann J, Duckers HJ, Schulte-Merker S
  • Journal Title: Development Volume: 142 Pages: 1695-1704
  • DOI: 10.1242/dev.117275
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Large gel proteomics-based analyses of the mechanisms for neuronal axon formation and guidance (2014)
  • Author(s): 馬場健太郎、浦崎明宏、稲垣直之
  • Journal Title: SEIBUTSU BUTSURI KAGAKU Volume: 58 Issue: 2 Pages: 49-52
  • DOI: 10.2198/sbk.58.49
  • NAID: 130004704685
  • ISSN: 0031-9082, 1349-9785
• [Presentation] 神経回路形成におけるSingarの役割の解析 (2016)
  • Author(s): 高野 拓郎、中澤 瞳、Colleen F. Manning、James S. Trimmer、河野 憲二、浦崎明宏、稲垣 直之
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
• [Presentation] Role of shootin1 during embryonic development in zebrafish (2014)
  • Author(s): A. Urasaki, T. Matsui, K. Kawakami, Y. Bessho, N. Inagaki
  • Organizer: The 2014 ASCB meeting
  • Place of Presentation: Philadelphia （USA）
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] ゼブラフィッシュを用いた脳形成におけるshootin1の役割 (2014)
  • Author(s): 浦崎明宏、渡瀬恵美子、松井貴輝、川上浩一、別所康全、稲垣直之
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂(奈良県奈良市)
  • Year and Date: 2014-06-11 – 2014-06-13