| Project/Area Number |
26840083
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 幹細胞 / 腸管発生 / 転写因子 / CDX2 / NEUROG3 / ヒトES細胞 / 再生医療 / 分化誘導 / 小腸上皮細胞 |
|---|
| Outline of Final Research Achievements |
We have induced human CDX2 in human embryonic stem (hES) cells, and examined their marker expression by immnohistochemistry, revealing unexpected result that showed clear reduction of intestinal epithelial cells after CDX2 induction. Our parallel transcriptome analysis also showed high similarity to neural cell lineage, compared with another adult tissues. We expected NEUROG3 as a downstream gene for CDX2, because it is a marker gene for intestinal secretion cells, but NEUROG3 induced hES cells were differentiated into functional neural cells that show calcium flux and action potential with multiple data from electrophysiological experiments. Finally we could obtain these new observations with a logical validity, although we didn't expect them in our initial plan.
|
