Search for novel protein kinases and protein phosphatases regulating cell division in plant cells
| Project/Area Number |
26840086
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hirosaki University |
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Principal Investigator |
Sasabe Michiko 弘前大学, 農学生命科学部, 准教授 (00454380)
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| Research Collaborator |
OWADA Rie
SOTA Harutoshi
MASUKO Yuki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 細胞分裂 / MAPK / CDK / プロテインキナーゼ / プロテインホスファターゼ / 細胞質分裂 / 植物 / リン酸化 / MAPキナーゼ / 細胞板 / 紡錘体 / フラグモプラスト / 微小管 |
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| Outline of Final Research Achievements |
To elucidate the molecular mechanisms of cell division in various organisms is important to reveal the developmental and differentiation processes in each organism. In this study, we aimed to identify the novel factors controlling plant cell division. The NACK-PQR pathway, which is a MAPK cascade unique to plant, is known as a key regulator of plant cytokinesis. This pathway is suppressed by CDKs during early M phase, and it is specifically activated during cytokinesis. Here we identified a novel type 2C protein phosphatase (PP2C) as a candidate that functions competitively to CDKs. This PP2C gene was dominantly expressed in the division zone of primary roots, emerging lateral roots and developing young leaves. Overexpression of this PP2C promoted the cell division but not the cell elongation in Arabidopsis plants, resulting in bigger seedlings with long roots. These results suggest this PP2C is involved in the cell division in plants.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Arabidopsis MAP65-4 Plays A Role in Phragmoplast Microtubule Organization and Marks the Cortical Cell Division Site.2017
Author(s)
Li, H., Sun, B., Sasabe, M., Deng, X., Machida, Y., Lin, H., Lee, Y., Liu, B.
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Journal Title
New Phytologist
Volume: -
Issue: 1
Pages: 187-201
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of Phosphoinositide-Binding Protein PATELLIN2 as a Substrate of Arabidopsis MPK4 MAP Kinase during Septum Formation in Cytokinesis.2016
Author(s)
Suzuki ,T., Matsushima, C., Nishimura, S., Higashiyama, T., Sasabe, M., Machida, Y
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Journal Title
Plant and Cell Physiology
Volume: 57
Issue: 8
Pages: 1744-1755
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site2015
Author(s)
Sasabe, M., Ishibashi, N., Haruta, T., Minami, A., Kurihara, D., Higashiyama, T., Nishihama, R., Ito, M., and Machida, Y.
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Journal Title
J. Plant Res.
Volume: 128
Issue: 2
Pages: 327-336
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 細胞板形成におけるNACK1キネシンの役割2016
Author(s)
鈴木伶奈, 森岡祉門, 西田結花, 桧垣匠, 植村知博, 安原裕紀, 馳澤盛一郎, 上田貴志, 町田泰則, 笹部美知子
Organizer
東北植物学会第6回大会
Place of Presentation
東北大学青葉山北キャンパス
Year and Date
2016-12-10
Related Report
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[Presentation] 植物の細胞板形成を支える分子メカニズム2015
Author(s)
笹部美知子, 桧垣匠, 大和田理恵, 相田治寿, 西田結花, 馳澤盛一郎, 町田泰則
Organizer
第56回日本植物生理学会年会
Place of Presentation
東京農業大学
Year and Date
2015-03-16 – 2015-03-19
Related Report
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