Screening for genes involved in the cold-induced organelle relocation using a T-DNA insertion library of the liverwort Marchantia polymorpha
| Project/Area Number |
26840088
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Utsunomiya University |
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Principal Investigator |
Kodama Yutaka 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (00455213)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | オルガネラ / 葉緑体 / 低温定位 / 寒冷定位 / T-DNA / ゼニゴケ / 葉緑体運動 / 植物細胞 / 低温応答 / フォトトロピン / 環境応答 / アガートラップ法 / 低温 / 寒冷定位運動 |
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| Outline of Final Research Achievements |
We developed a simplified Agrobacterium-mediated transformation technique (AgarTrap method) for the liverwort Marchantia polymorpha, and produced a T-DNA insertion mutant library containing over 10 thousands lines. Using the mutant library, we explored mutant(s) deficient in the organelle relocation movement response, and successfully found one mutant. Based on various experiments, we found that the mutant lacks a novel gene, but not known genes involved in the response. In our future work, we will analyze the novel gene.
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Report
(4 results)
Research Products
(31 results)
