| Project/Area Number |
26840094
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Miyashima Shunsuke 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (20727169)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胚発生 / MicroRNA / シロイヌナズナ / 放射パターン |
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| Outline of Final Research Achievements |
The formation of radially symmetric tissue pattern, which is one of the most basic processes in the development of vascular plants, is initiated during embryogenesis. Our previous works revealed that MIR165 and MIR166 genes, a mobile small RNA controlling radial pattern formation in Arabidopsis, exhibit the tissue-specific expression pattern during embryognenesis. To understand the molecular mechanism initiating the radial patterning during embryogenesis, we aimed to identify cis- and trans-factors regulating MIR165/166 expression during early embryogenesis.
As a result, we clarified two major cis-elements on MIR165/166 promoters determining the prodoerem-specific expression of MIR165/166 genes in the lower tier. In addition to that, two types of transcription factors were identified as candidates acting on these cis-elements in MIR165/166 promoter.
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