A genetic framework for the control of DNA polyploidization in plants

• Project/Area Number: 26840096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Takahashi Naoki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40553623)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 細胞周期 / エンドサイクル / 環境ストレス / 植物ホルモン / CDK / 根

Outline of Final Research Achievements

Plant growth is controlled by the timing of transition from mitotic cell cycle to endoreplication. Therefore, to understand plant growth under fluctuating environmental conditions, it is important to know the molecular mechanism of endoreplication onset in plants. Previous studies reveal that CCS52A1, an activator of an E3 ligase, anaphase-promoting complex/cyclosome (APC/C), is involved in the control of endoreplication onset. Our study revealed that the induction of CCS52A1 gene is regulated by tissue-specific activation of cytokinin biosynthesis in response to environmental stress.

Research Products

• [Journal Article] The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. (2016)
  • Author(s): Weimer, A.K., Biedermann, S., Harashima, H., Roodbarkelari, F., Takahashi, N., Foreman, J., Guan, Y., Pochon, G., Heese, M., Van Damme, D., Sugimoto, K., Koncz, C., Doerner, P., Umeda, M., Schnittger, A.
  • Journal Title: EMBO J Volume: 35 Issue: 19 Pages: 2068-2086
  • DOI: 10.15252/embj.201593083
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] DNA damage inhibits lateral root formation by upregulating cytokinin biosynthesis genes in Arabidopsis thaliana. (2016)
  • Author(s): Davis, M.M., Ogita, N., Inagaki, S., Takahashi, N., Umeda, M.
  • Journal Title: Genes Cells Volume: 21 Issue: 11 Pages: 1195-1208
  • DOI: 10.1111/gtc.12436
  • NAID: 120006537391
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 根端メリステムのサイズ制御機構 (2016)
  • Author(s): 高橋直紀、梅田正明
  • Journal Title: BSJ-Review Volume: 7 Pages: 201-209
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 植物のDNA損傷応答 (2016)
  • Author(s): 高橋直紀、梅田正明
  • Journal Title: 生物と化学 Volume: 54 Pages: 123-129
  • NAID: 130005289841
• [Journal Article] Cytokinins promote onset of endoreplication by controlling cell cycle machinery (2014)
  • Author(s): Takahashi, N. and Umeda, M.
  • Journal Title: Plant Signal. Behav. Volume: 9 Issue: 8 Pages: e29396-e29396
  • DOI: 10.4161/psb.29396
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Arabidopsis SIAMESE-RELATED Cyclin-Dependent Kinase Inhibitors SMR5 and SMR7 Regulate the DNA Damage Checkpoint in Response to Reactive Oxygen Species (2014)
  • Author(s): Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama Okamoto K, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L
  • Journal Title: Plant Cell Volume: 26 Issue: 1 Pages: 296-309
  • DOI: 10.1105/tpc.113.118943
  • Peer Reviewed
• [Presentation] Brassinosteroids are involved in stem cell replenishment in Arabidopsis roots under DNA damage (2017)
  • Author(s): Naoki Takahashi, Masaaki Umeda
  • Organizer: Plant Organ Growth Symposium
  • Place of Presentation: Elche, Spain
  • Year and Date: 2017-03-15
  • Int'l Joint Research
• [Presentation] Plant Stem Cells Undergo Cell Death to Maintain Genome Integrity (2016)
  • Author(s): Naoki Takahashi, Masaaki Umeda
  • Organizer: 10th 3R Symposium
  • Place of Presentation: ホテル一畑・島根県松江市
  • Year and Date: 2016-11-13
  • Int'l Joint Research
• [Presentation] DNA damage response in Arabidopsis roots (2016)
  • Author(s): Naoki Takahashi, Masaaki Umeda
  • Organizer: Plant Genome Stability and Change 2016
  • Place of Presentation: 湘南国際村センター・神奈川県横須賀市
  • Year and Date: 2016-07-07
  • Int'l Joint Research
• [Presentation] DNA double-strand breaks suppress root growth by enhancing cytokinin biosynthesis in Arabidopsis thaliana (2016)
  • Author(s): Naoki Takahashi, Masaaki Umeda
  • Organizer: Plant Genome Stability and Change 2016
  • Place of Presentation: 湘南国際村センター・神奈川県三浦郡葉山町
  • Year and Date: 2016-07-07
  • Int'l Joint Research
• [Presentation] Genome Integrity in root stem cells under DNA stress (2016)
  • Author(s): Naoki Takahashi, Keisuke Fujimoto, Masaaki Umeda
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学・岩手県盛岡市
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] 根端分裂組織のサイズ制御機構 (2015)
  • Author(s): 高橋直紀、丸池加奈子、高塚大知、梅田正明
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ・新潟県新潟市
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] Stem cell replenishment maintains genome integrity (2015)
  • Author(s): Umeda M, Fujimoto K, Takahashi N
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] DNA損傷応答に関わる転写因子SOG1の標的遺伝子の解析 (2015)
  • Author(s): 荻田伸夫、奥島葉子、倉田哲也、時澤睦朋、山本義治、高橋直紀、梅田正明
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Regulation of auxin signaling is essential for stem cell maintenance under DNA stress conditions (2015)
  • Author(s): Takahashi N, Umeda M
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Brassinosteroids are involved in stem cell replenishment in Arabidopsis roots under DNA stress conditions (2015)
  • Author(s): Fujimoto K, Takahashi N, Umeda M
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] DNA damage response in Arabidopsis roots (2014)
  • Author(s): Takahashi N, Chen P, Umeda M
  • Organizer: Plant Genome Stability and Change 2014
  • Place of Presentation: California, USA
  • Year and Date: 2014-07-17 – 2014-07-20
• [Book] Cell Cycle: Cell Biology (2014)
  • Author(s): Takahashi N, Umeda M
  • Total Pages: 19
  • Publisher: Springer