| Project/Area Number |
26840099
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Research Collaborator |
HARADA Jiro 久留米大学, 医化学講座, 講師
FUJITA Yuichi 名古屋大学, 生命農学研究科, 教授
YAMAMOTO Haruki 大阪大学, 蛋白質研究所, JSPS研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | バクテリオクロロフィル / 光合成 / 色素 / 近赤外光 / 光合成の進化 / 光合成細菌 / 生合成酵素 |
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| Outline of Final Research Achievements |
Chlorophyllide oxidoreductase (COR) is important for the formation of a bacteriochlorin ring, and is a unique pigment biosynthesis enzyme which shows distinct substrate recognition and hydrogen addition activities, depending on the host strains. We found factors essential for making a model photosynthetic bacterium produce bacteriochlorophyll-b which absorbs near-infrared light. The important factors are the loss of functions of both intrinsic COR and 8-vinyl reductase and the genetic incorporation of the BchYZ catalytic components into the host strain. We are further analyzing substrate recognition of COR in detail and enzymatic activities of other pigment biosynthesis enzymes.
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