Auxin network spatiotemporally orchestrating stem cell initiation
Project/Area Number |
26840101
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Hiroshima Institute of Technology (2016-2017) National Institute for Basic Biology (2014-2015) |
Principal Investigator |
IMAI Akihiro 広島工業大学, 生命学部, 助教 (40711198)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | オーキシン / 幹細胞 / 発光イメージング / 低分子RNA / ヒメツリガネゴケ |
Outline of Final Research Achievements |
In Physcomitrella patens, a leaf detachment induces cell reprogramming in which leaf cells are changed to stem cells. Transgenic plants defect in auxin signaling genes showed the reprogramming efficiency. In this study, to reveal the dynamics of auxin accumulation and auxin signaling factors, auxin dual-luc sensors and small RNA sensors were developed. The results suggested that leaf detachment triggers decrease in the small RNAs first and then induces a transient increase in auxin level and PpARF protein, which leads to cell reprogramming from a leaf cell to a stem cell.
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Report
(5 results)
Research Products
(10 results)
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[Journal Article] A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens2017
Author(s)
Chen Li, Yusuke Sako, Akihiro Imai, Tomoaki Nishiyama, Kari Thompson, Minoru Kubo, Yuji Hiwatashi, Yukiko Kabeya, Dale Karlson, Shu-Hsing Wu, Masaki Ishikawa, Takashi Murata, Philip N. Benfey, Yoshikatsu Sato, Yosuke Tamada, Mitsuyasu Hasebe
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 14242-14242
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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