| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In Physcomitrella patens, a leaf detachment induces cell reprogramming in which leaf cells are changed to stem cells. Transgenic plants defect in auxin signaling genes showed the reprogramming efficiency. In this study, to reveal the dynamics of auxin accumulation and auxin signaling factors, auxin dual-luc sensors and small RNA sensors were developed. The results suggested that leaf detachment triggers decrease in the small RNAs first and then induces a transient increase in auxin level and PpARF protein, which leads to cell reprogramming from a leaf cell to a stem cell.
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