| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In the flowering plants, sexual reproduction begins with pollination, the transfer of pollen to stigma of the pistil. After pollen germination, pollen tube elongates in the pistil, are attracted to the ovules (ovule develops into a seed), and finally, fertilization occurs. This phenomenon is called as 'pollen tube guidance'. This process is an important in the reproductive process, however, the molecular mechanisms of how pollen tube grows toward the ovule is largely unknown.
Here we attempted to visualize pollen tube growth and guidance inside the clearling pistil of the Arabidopsis thaliana by the two-photon microscopy. Addition to that, the method for live imaging inside the living pistil was developed. As a result, I succeeded to deep and live imaging of pollen tube growth and guidance in the pistil.
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