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Analysis of environmental control of a sex-determininng gene in crustacean Daphnia magna

Research Project

Project/Area Number 26840105
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionOsaka University

Principal Investigator

Kato Yasuhiko  大阪大学, 工学(系)研究科(研究院), 助教 (60415932)

Research Collaborator NAKANISHI Takashi  
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords環境性決定 / 幼若ホルモン / RNA-Seq / doublesex 遺伝子 / FTZ-F1 / ミジンコ / TALEN / 2A ペプチド / 性決定 / 幼若ホルモン受容体 MET / ゲノム編集技術 / オオミジンコ / dsx 遺伝子 / オオミジンコ / 「ゲノム支援」活動
Outline of Final Research Achievements

Sex-determining systems can be broadly divided into two categories: genetic and environmental. We previously identified a gene that regulates environmental sex determination in Daphnia. Interestingly this gene was homologous to doublesex genes that are regulators of genetic sex determination, suggesting that there is a previously unidentified link between genetic and environmental sex determination. In this study, we aimed to find factors that control doublesex gene expression of Daphnia. RNA-seq analysis and comparative studies of the genes involved in sex determination enabled us to find candidates of regulators of doublesex gene.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] TALEN-mediated homologous recombination in Daphnia magna.2015

    • Author(s)
      Nakanishi T, Kato Y, Matsuura T, Watanabe H
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 18312-18312

    • DOI

      10.1038/srep18312

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna2015

    • Author(s)
      内藤彰子、加藤泰彦、中西貴士、松浦友亮、渡邉肇
    • Journal Title

      Biology OPEN

      Volume: 13 Issue: 3 Pages: 364-369

    • DOI

      10.1242/bio.20149738

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Use of the bicistronic expression involving viral T2A peptide in Daphnia magna2016

    • Author(s)
      Hitoshi Kumagai, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      第62回日本生態学会大会
    • Place of Presentation
      仙台
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of Transcription Activation of the Environmental Sex Determining Gene Doublesex1 in Daphnia magna2015

    • Author(s)
      Syafiqah Ishak, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Eye Color Gene Scarlet as a Visible Marker for Daphnia magna Transgenesis2015

    • Author(s)
      Nur Izzatur Binti Ismail, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Use of the bicistronic expression involving viral T2A peptide in Daphnia magna2015

    • Author(s)
      Hitoshi Kumagai, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Efficient DNA integration into germ line genome via TALEN in Daphnia magna2015

    • Author(s)
      Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      Conference on Transposition and Genome Engineering 2015
    • Place of Presentation
      奈良
    • Year and Date
      2015-11-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミジンコのゲノム操作による環境性決定メカニズムの解明2015

    • Author(s)
      加藤泰彦、中西貴士、渡邉肇
    • Organizer
      「細胞を創る」研究会8.0
    • Place of Presentation
      大阪
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 環境指標生物オオミジンコにおけるTALENを用いた外来遺伝子導入技術の開発2015

    • Author(s)
      中西貴士、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      日本生物工学会 若手会2015
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Efficient DNA integration into germ line genome via programmable nucleases in Daphnia magna2015

    • Author(s)
      Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      Cold Spring Harbor Laboratory Meetings & Courses Program "GENOME ENGINEERING: THE CRISPR/CAS REVOLUTION"
    • Place of Presentation
      New York, USA
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An lncRNA regulates an environmental sex determining gene doublesex1 in Daphnia2014

    • Author(s)
      加藤泰彦
    • Organizer
      16th Tokyo RNA Club
    • Place of Presentation
      東京大学 武田ホール(東京都)
    • Year and Date
      2014-11-28
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna2014

    • Author(s)
      内藤彰子、加藤泰彦、中西貴士、松浦友亮、渡邉肇
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] CRISPR/Cas9、TALENを利用したオオミジンコのゲノム編集技術の開発2014

    • Author(s)
      中西貴士、内藤彰子、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] CRISPR/CasおよびTALENによるDaphnia magnaのゲノム編集技術の開発2014

    • Author(s)
      内藤彰子、中西貴士、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-09
    • Related Report
      2014 Research-status Report
  • [Presentation] An lncRNA regulates an environmental sex determining gene doublesex1 in Daphnia2014

    • Author(s)
      加藤泰彦、須藤優海、渡邉肇
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウィンクあいち(愛知県)
    • Year and Date
      2014-07-23
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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