Analysis of environmental control of a sex-determininng gene in crustacean Daphnia magna

• Project/Area Number: 26840105
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Osaka University
• Principal Investigator: Kato Yasuhiko 大阪大学, 工学(系)研究科(研究院), 助教 (60415932)
• Research Collaborator: NAKANISHI Takashi
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 環境性決定 / 幼若ホルモン / RNA-Seq / doublesex 遺伝子 / FTZ-F1 / ミジンコ / TALEN / 2A ペプチド / 性決定 / 幼若ホルモン受容体 MET / ゲノム編集技術 / オオミジンコ / dsx 遺伝子 / オオミジンコ / 「ゲノム支援」活動

Outline of Final Research Achievements

Sex-determining systems can be broadly divided into two categories: genetic and environmental. We previously identified a gene that regulates environmental sex determination in Daphnia. Interestingly this gene was homologous to doublesex genes that are regulators of genetic sex determination, suggesting that there is a previously unidentified link between genetic and environmental sex determination. In this study, we aimed to find factors that control doublesex gene expression of Daphnia. RNA-seq analysis and comparative studies of the genes involved in sex determination enabled us to find candidates of regulators of doublesex gene.

Research Products

• [Journal Article] TALEN-mediated homologous recombination in Daphnia magna. (2015)
  • Author(s): Nakanishi T, Kato Y, Matsuura T, Watanabe H
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 18312-18312
  • DOI: 10.1038/srep18312
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna (2015)
  • Author(s): 内藤彰子、加藤泰彦、中西貴士、松浦友亮、渡邉肇
  • Journal Title: Biology OPEN Volume: 13 Issue: 3 Pages: 364-369
  • DOI: 10.1242/bio.20149738
  • Peer Reviewed / Open Access
• [Presentation] Use of the bicistronic expression involving viral T2A peptide in Daphnia magna (2016)
  • Author(s): Hitoshi Kumagai, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: 第62回日本生態学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-20
• [Presentation] Analysis of Transcription Activation of the Environmental Sex Determining Gene Doublesex1 in Daphnia magna (2015)
  • Author(s): Syafiqah Ishak, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Eye Color Gene Scarlet as a Visible Marker for Daphnia magna Transgenesis (2015)
  • Author(s): Nur Izzatur Binti Ismail, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Use of the bicistronic expression involving viral T2A peptide in Daphnia magna (2015)
  • Author(s): Hitoshi Kumagai, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Efficient DNA integration into germ line genome via TALEN in Daphnia magna (2015)
  • Author(s): Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: Conference on Transposition and Genome Engineering 2015
  • Place of Presentation: 奈良
  • Year and Date: 2015-11-17
  • Int'l Joint Research
• [Presentation] ミジンコのゲノム操作による環境性決定メカニズムの解明 (2015)
  • Author(s): 加藤泰彦、中西貴士、渡邉肇
  • Organizer: 「細胞を創る」研究会8.0
  • Place of Presentation: 大阪
  • Year and Date: 2015-11-13
  • Invited
• [Presentation] 環境指標生物オオミジンコにおけるTALENを用いた外来遺伝子導入技術の開発 (2015)
  • Author(s): 中西貴士、加藤泰彦、松浦友亮、渡邉肇
  • Organizer: 日本生物工学会 若手会2015
  • Place of Presentation: 鹿児島
  • Year and Date: 2015-10-27
• [Presentation] Efficient DNA integration into germ line genome via programmable nucleases in Daphnia magna (2015)
  • Author(s): Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
  • Organizer: Cold Spring Harbor Laboratory Meetings & Courses Program "GENOME ENGINEERING: THE CRISPR/CAS REVOLUTION"
  • Place of Presentation: New York, USA
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] An lncRNA regulates an environmental sex determining gene doublesex1 in Daphnia (2014)
  • Author(s): 加藤泰彦
  • Organizer: 16th Tokyo RNA Club
  • Place of Presentation: 東京大学 武田ホール（東京都）
  • Year and Date: 2014-11-28
  • Invited
• [Presentation] Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna (2014)
  • Author(s): 内藤彰子、加藤泰彦、中西貴士、松浦友亮、渡邉肇
  • Organizer: 第37回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-11-25
• [Presentation] CRISPR/Cas9、TALENを利用したオオミジンコのゲノム編集技術の開発 (2014)
  • Author(s): 中西貴士、内藤彰子、加藤泰彦、松浦友亮、渡邉肇
  • Organizer: 第37回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-11-25
• [Presentation] CRISPR/CasおよびTALENによるDaphnia magnaのゲノム編集技術の開発 (2014)
  • Author(s): 内藤彰子、中西貴士、加藤泰彦、松浦友亮、渡邉肇
  • Organizer: 第66回日本生物工学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-09-09
• [Presentation] An lncRNA regulates an environmental sex determining gene doublesex1 in Daphnia (2014)
  • Author(s): 加藤泰彦、須藤優海、渡邉肇
  • Organizer: 第16回日本RNA学会年会
  • Place of Presentation: ウィンクあいち（愛知県）
  • Year and Date: 2014-07-23