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The mechanism for sex reversal in ovarian aromatase loss-of-function mutant medaka

Research Project

Project/Area Number 26840107
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Nakamoto Masatoshi  東京海洋大学, 学術研究院, 博士研究員 (80447721)

Research Collaborator NAGAHAMA Yoshitaka  愛媛大学, 社会連携機構, 教授
Young Graham  University of Washington, School of Aquatic and Fishery Sciences, 教授
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords性分化 / 性転換 / 性的可塑性 / メダカ / エストロゲン / 卵巣分化 / 卵巣形成 / 硬骨魚類 / 魚類 / 内分泌学 / 分子生物学
Outline of Final Research Achievements

Although estrogens have been generally considered to play a critical role in ovarian differentiation in non-mammalian vertebrates, the specific functions of estrogens during ovarian differentiation remain unclear. We isolated two mutants with premature stops in the ovarian aromatase (cyp19a1) gene from a mutagenesis library of the medaka, Oryzias latipes. In XX mutants, gonads first differentiated into normal ovaries containing many ovarian follicles that failed to accumulate yolk. Subsequently, ovarian tissues underwent extensive degeneration, followed by the appearance of testicular tissues. During the initial phase of degeneration of ovarian tissues, the genes involved in synthesis of non-aromatizable androgens were up-regulated. Our results suggest that the imbalance of estrogens and androgens, especially the potential for an increase in androgens could be part of the mechanism that induced degeneration of ovarian follicles in ovaries and sex reversal of cyp19a1 mutants.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2018

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Ovarian aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty2018

    • Author(s)
      Nakamoto Masatoshi、Shibata Yasushi、Ohno Kaoru、Usami Takeshi、Kamei Yasuhiro、Taniguchi Yoshihito、Todo Takeshi、Sakamoto Takashi、Young Graham、Swanson Penny、Naruse Kiyoshi、Nagahama Yoshitaka
    • Journal Title

      Mol Cell Endocrinol

      Volume: 460 Pages: 104-122

    • DOI

      10.1016/j.mce.2017.07.013

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

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