| Project/Area Number |
26840108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
Osugi Tomohiro 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 研究員 (50507986)
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| Research Collaborator |
SHIRAISHI Akira 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 研究員
SATAKE Honoo 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 主幹研究員
SUGAHARA Kohtaro 公益財団法人サントリー生命科学財団, 生物有機科学研究所・構造生命科学研究部, 研究員
YAMAGAKI Tohru 公益財団法人サントリー生命科学財団, 生物有機科学研究所・構造生命科学研究部, 主席研究員
SUEMATSU Makoto 慶応義塾大学, 医学部, 教授
KAGEYAMA Akira 神戸大学, 大学院システム情報学研究科, 教授
SAKAMOTO Naohisa 神戸大学, 大学院システム情報学研究科, 講師
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ホヤ / 神経ペプチド / 質量分析イメージング |
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| Outline of Final Research Achievements |
In this study, we analyzed the distribution of neuropeptides in the Ciona brain using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) imaging mass spectrometry (MS) based on our previous peptidomics data. Major Ciona neuropeptides were detected on a single brain section, and the images of their distributions were acquired with a spatial resolution of 20 micro meters. We further developed a method for high-resolution imaging MS by a combination of fluorescent microscope and imaging MS in the brain sections of transgenic Ciona that expresses fluorescent protein in neurons. The high-resolution imaging MS for the first time showed the distribution of four peptides in a single brain section at single-cell resolution. Both the high-resolution imaging MS and immunohistochemistry using specific antiserum against the neuropeptide Ci-TK-I yielded similar results, suggesting that the high-resolution imaging MS has a potential to serve as a new platform for histology.
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