| Project/Area Number |
26840114
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Genetics/Chromosome dynamics
|
|---|
| Research Institution | The University of Tokyo (2015-2017)
National Institute of Genetics (2014) |
|---|
Principal Investigator |
Akamatsu Yufuko 東京大学, 分子細胞生物学研究所, 助教 (50381661)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | リボソームRNA遺伝子 / DNA複製 / ゲノム安定性 / RNA poymerase I / 転写 / ゲノム不安定性 / rRNA遺伝子 / DNA複製阻害点 |
|---|
| Outline of Final Research Achievements |
In S phase, the replication and transcription of genomic DNA need to accommodate each other, otherwise their machineries collide, with chromosomal instability as a possible consequence. The ribosomal RNA gene (rDNA) is the most actively transcribed gene in human cells and the activity is high during S phase. To understand how the collision between rDNA transcription and replication is prevented, the role of replication fork barrier (RFB) was investigated. We found that the Sal-box elements that are able to terminate rDNA transcription were the cis elements for the RFB activity. When fork arrests at these sites failed, rDNA transcription impedes replication fork progression. These results reveal a role of RFB for coordinating the progression of replication and transcription activity in highly transcribed rDNA.
|
