Genome sequencing and transgenesis of a heteropteran insect Nysius plebeius for studying bacteriocyte-associated symbiosis
| Project/Area Number |
26840116
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Evolutionary biology
|
|---|
| Research Institution | University of the Ryukyus (2015-2016)
Hokkaido University (2014) |
|---|
Principal Investigator |
MATSUURA Yu 琉球大学, 熱帯生物圏研究センター, 助教 (80723824)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 細胞内共生 / ヒメナガカメムシ / 菌細胞 / ゲノム / 共生細菌 / 栄養代謝 / 遺伝子機能 / de novo アセンブル / Oncopeltus fasciatus / アノテーション / 一過性発現系 |
|---|
| Outline of Final Research Achievements |
Preliminary genome sequencing and transgenic experiments of Nysius plebeius were performed in order to elucidate genetic and developmental basis of bacteriocyte-associated symbiosis independently evolved in small groups of heteropteran insects. With illumina-based paired end and mate pair library sequencing and de novo assembly using Platanus, we have generated genome scaffolds nearly full length of the insect genome as well as identifying immunity related genes and nutritional inter-dependence between the host and symbiont. On the other hand, new manual methods were established for injecting various foreign nucleic acids such as plasmid, mRNA and gRNA into early embryos of N. plebeius, although I have not succeeded in obtaining any transgenic or knockout lines so far. The study will continue based on these genetic and experimental resources for understanding the evolutionary basis of bacteriocyte in N. plebeius.
|
Report
(4 results)
Research Products
(27 results)
-
-
-
-
[Journal Article] Gut symbiotic bacteria stimulate insect growth and egg production by modulating hexamerin and vitellogenin gene expression2017
Author(s)
Lee JB, Park KE, Lee SA, Jang SH, Eo HJ, Jang HA, Kim CH, Ohbayashi T, Matsuura Y, Kikuchi Y, Futahashi R, Fukatsu T, and Lee BL
-
Journal Title
Developmental and comparative immunology
Volume: 69
Pages: 12-22
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
[Journal Article] Intrasperm vertical symbiont transmission.2014
Author(s)
Watanabe K., Yukuhiro F., Matsuura Y., Fukatsu T., Noda H.
-
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 111
Issue: 20
Pages: 7433-7437
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
[Presentation] Bacteriocyte development regulated by Hox genes in an insect, Nysius plebeius2015
Author(s)
Matsuura, Y., Kikuchi, Y., Miura, T., Fukatsu, T.
Organizer
Gordon Research Conference, Animal-Microbe Symbioses
Place of Presentation
Waterville Valley（New Hampshire, USA）
Year and Date
2015-05-22
Related Report
Int'l Joint Research
-
-
-
-
