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Genome sequencing and transgenesis of a heteropteran insect Nysius plebeius for studying bacteriocyte-associated symbiosis

Research Project

Project/Area Number 26840116
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionUniversity of the Ryukyus (2015-2016)
Hokkaido University (2014)

Principal Investigator

MATSUURA Yu  琉球大学, 熱帯生物圏研究センター, 助教 (80723824)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細胞内共生 / ヒメナガカメムシ / 菌細胞 / ゲノム / 共生細菌 / 栄養代謝 / 遺伝子機能 / de novo アセンブル / Oncopeltus fasciatus / アノテーション / 一過性発現系
Outline of Final Research Achievements

Preliminary genome sequencing and transgenic experiments of Nysius plebeius were performed in order to elucidate genetic and developmental basis of bacteriocyte-associated symbiosis independently evolved in small groups of heteropteran insects. With illumina-based paired end and mate pair library sequencing and de novo assembly using Platanus, we have generated genome scaffolds nearly full length of the insect genome as well as identifying immunity related genes and nutritional inter-dependence between the host and symbiont. On the other hand, new manual methods were established for injecting various foreign nucleic acids such as plasmid, mRNA and gRNA into early embryos of N. plebeius, although I have not succeeded in obtaining any transgenic or knockout lines so far. The study will continue based on these genetic and experimental resources for understanding the evolutionary basis of bacteriocyte in N. plebeius.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 4 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] University of Montana(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Bayreuth(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Pusan National University(韓国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Gut symbiotic bacteria stimulate insect growth and egg production by modulating hexamerin and vitellogenin gene expression2017

    • Author(s)
      Lee JB, Park KE, Lee SA, Jang SH, Eo HJ, Jang HA, Kim CH, Ohbayashi T, Matsuura Y, Kikuchi Y, Futahashi R, Fukatsu T, and Lee BL
    • Journal Title

      Developmental and comparative immunology

      Volume: 69 Pages: 12-22

    • DOI

      10.1016/j.dci.2016.11.019

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Obligate gut symbiotic association in the sloe bug Dolycoris baccarum (Hemiptera: Pentatomidae)2017

    • Author(s)
      Itoh H., Matsuura Y., Hosokawa T., Fukatsu T., Kikuchi Y.
    • Journal Title

      Applied Entomology and Zoology

      Volume: 52 Issue: 1 Pages: 51-59

    • DOI

      10.1007/s13355-016-0453-0

    • NAID

      40021101445

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Recurrent evolution of gut symbiotic bacteria in pentatomid stinkbugs2016

    • Author(s)
      Hosokawa T., Matsuura Y., Kikuchi Y., Fukatsu T.
    • Journal Title

      Zoological Letters

      Volume: 2 Issue: 1 Pages: 34-34

    • DOI

      10.1186/s40851-016-0061-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Phylogenetically Diverse <i>Burkholderia</i> Associated with Midgut Crypts of Spurge Bugs, <i>Dicranocephalus</i> spp. (Heteroptera: Stenocephalidae)2016

    • Author(s)
      Stefan Martin Kuechler, 松浦優, Konrad Dettner, 菊池義智
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 2 Pages: 145-153

    • DOI

      10.1264/jsme2.ME16042

    • NAID

      130005158505

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] セミ類の共生菌と冬虫夏草.2016

    • Author(s)
      深津武馬・松浦優・棚橋薫彦・森山実.
    • Journal Title

      冬虫夏草

      Volume: 36 Pages: 13-17

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Ultrabithorax is essential for bacteriocyte development2015

    • Author(s)
      Yu Matsuura, Yoshitomo Kikuchi, Toru Miura, Takema Fukatsu
    • Journal Title

      Proceedings of the National Academy of Sciences of U.S.A.

      Volume: 112 Issue: 30 Pages: 9376-9381

    • DOI

      10.1073/pnas.1503371112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Burkholderia of Plant-Beneficial Group are Symbiotically Associated with Bordered Plant Bugs (Heteroptera: Pyrrhocoroidea: Largidae)2015

    • Author(s)
      Kazutaka Takeshita, Yu Matsuura, Hideomi Itoh, Ronald Navarro, Tomoyuki Hori, Teruo Sone, Yoichi Kamagata, Peter Mergaert, Yoshitomo Kikuchi
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 4 Pages: 321-329

    • DOI

      10.1264/jsme2.me15153

    • NAID

      130005116033

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Infection prevalence of Sodalis symbionts among stinkbugs.2015

    • Author(s)
      Hosokawa T., Kaiwa N., Matsuura Y., Kikuchi Y., Fukatsu T.
    • Journal Title

      Zoological Letters

      Volume: 1 Issue: 1 Pages: 1-7

    • DOI

      10.1186/s40851-014-0009-5

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 菌細胞の獲得と進化:ナガカメムシ類における菌細胞の多様性と発生学的起源2014

    • Author(s)
      松浦 優
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 83 Pages: 223-229

    • NAID

      130005063991

    • Related Report
      2014 Research-status Report
  • [Journal Article] Intrasperm vertical symbiont transmission.2014

    • Author(s)
      Watanabe K., Yukuhiro F., Matsuura Y., Fukatsu T., Noda H.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 111 Issue: 20 Pages: 7433-7437

    • DOI

      10.1073/pnas.1402476111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Diverse patterns of symbiotic organ formation during embryonic development in lygaeoid bugs (Heteroptera: Lygaeoidea)2017

    • Author(s)
      Kuechler S.M., Matsuura Y., Koga R., Dettner K., Fukatsu T.
    • Organizer
      FEMS 2017, 7th Congress of European Microbiologists
    • Place of Presentation
      Feria ValenciaーConvention & Exhibition Centre(Valencia, Spain)
    • Year and Date
      2017-07-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] セミー細菌ー真菌による3者複合共生系の進化.2017

    • Author(s)
      松浦優・森山実・Lukasik Piotr・棚橋薫彦・孟憲英・Vanderpool Dan・菊池義智・McCutcheon John・ 深津武馬.
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒョウタンナガカメムシ類で見つかった環境獲得型細胞内共生.2017

    • Author(s)
      竹下和貴・松浦優・孟憲英・三谷恭雄・新里尚也・菊池義智
    • Organizer
      第61回日本応用動物昆虫学会大会.
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] おなかの中ではちがう顔~Burkholderiaの形がホソヘリカメムシ共生時に変化するのはなぜ?.2017

    • Author(s)
      後藤栞・竹下和貴・大林翼・松浦優・菊池義智
    • Organizer
      第61回日本応用動物昆虫学会大会.
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Transcriptomic dissection of bacteriocyte symbiosis in a hemipiteran insect, Nysius plebeius.2016

    • Author(s)
      Matsuura Y., Ogawa K., Matsunami M., Kikuchi Y., Shigenobu S., Fukatsu T., Miura T.
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology and the 87th meeting of Zoological Society of Japan.
    • Place of Presentation
      Okinawa Convention Center (沖縄県宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 環境から獲得される細胞内共生細菌:ナガカメムシ類で見つかった腸内共生から細胞内共生へのミッシングリンク.2016

    • Author(s)
      竹下和貴・松浦優・孟憲英・三谷恭雄・菊池義智
    • Organizer
      日本微生物生態学会第31回大会.
    • Place of Presentation
      横須賀市文化会館.(神奈川県横須賀市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] Burkholderiaのヒミツの姿~Burkholderia細胞壁合成不全変異株はホソヘリカメムシ共生時に異常な細胞形態を示す.2016

    • Author(s)
      後藤栞・竹下和貴・大林翼・松浦優・菊池義智
    • Organizer
      日本微生物生態学会第31回大会.
    • Place of Presentation
      横須賀市文化会館.(神奈川県横須賀市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒメナガカメムシ菌細胞の形成に関わる遺伝子群の探索2016

    • Author(s)
      松浦優, 小川浩太, 松波雅俊, 菊池義智, 重信秀治, 深津武馬, 三浦徹
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用昆虫学会大会 合同大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府堺市)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Hox 遺伝子が司る共生器官の発生 ーナガカメムシにおける菌細胞の進化ー2015

    • Author(s)
      松浦優
    • Organizer
      日本進化学会第17回東京大会
    • Place of Presentation
      中央大学後楽園キャンパス(東京都文京区)
    • Year and Date
      2015-08-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Bacteriocyte development regulated by Hox genes in an insect, Nysius plebeius2015

    • Author(s)
      Matsuura, Y., Kikuchi, Y., Miura, T., Fukatsu, T.
    • Organizer
      Gordon Research Conference, Animal-Microbe Symbioses
    • Place of Presentation
      Waterville Valley(New Hampshire, USA)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒメナガカメムシ菌細胞内共生細菌のゲノム解析2015

    • Author(s)
      松浦優, 竹下和貴, 前田太郎, 三浦徹,重信秀治,菊池義智, 深津武馬
    • Organizer
      第59回日本応用昆虫学会大会
    • Place of Presentation
      山形大学小白川キャンパス
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] A Hox gene is essential for bacteriocyte development and localization of symbionts in an insect Nysius plebeius2014

    • Author(s)
      Matsuura, Y.
    • Organizer
      Open Seminar at Instituto Cavanilles de Biodiversidad y Biologia Evolutiva
    • Place of Presentation
      University of Valencia, Spain
    • Year and Date
      2014-10-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Development of symbiotic organ: Hox genes regulate development of bacteriome and localization of bacterial symbiont in seed bug Nysius plebeius2014

    • Author(s)
      Matsuura Y., Kikuchi Y., Koga R., Miura T., and Fukatsu T.
    • Organizer
      The fifth meeting of the European Society for Evolutionary Developmental Biology
    • Place of Presentation
      University of Vienna, Austria
    • Year and Date
      2014-07-25
    • Related Report
      2014 Research-status Report
  • [Remarks] 産業技術総合研究所プレスリリース「昆虫の共生のための細胞がどのようにできるかを解明」

    • URL

      http://www.aist.go.jp/aist_j/press_release/pr2015/pr20150714/pr20150714.html

    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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