Functional diversification process of juvenile hormone pathway through the acquisition of novel regulatory relationships

• Project/Area Number: 26840122
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Utsunomiya University (2015); Okazaki Research Facilities, National Institutes of Natural Sciences (2014)
• Principal Investigator: Miyakawa Hitoshi 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (30631436)
• Research Collaborator: IGUCHI Taisen ; MIYAGAWA Shinichi ; OGINO Yukiko ; HIRUTA Chizue ; TOYOTA Kenji ; SUMIYA Eri
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 幼若ホルモン / Methoprene-tolerant / 節足動物 / ミジンコ / リガンド特異性

Outline of Final Research Achievements

Over the course of evolutionary time, juvenile hormone (JH) and its signaling pathway have acquired novel functions, both flexibly and repeatedly, while retaining their core roles related to the arthropod life cycle. To elucidate the process of functional diversification of JH, first, I compared the ligand selectivity of the JH receptor, Met, against four in four arthropod species (i.e., three insects: Drosophila, Aedes, Tribolium, and one crustacean: Daphnia) by performing a two-hybrid luciferase assay with Met and SRC orthologs. Obtained results suggest that the sequence structure of Met affects its ligand sensitivity. Next, I compared downstream signal cascade of Met between insect and crustacean species, and demonstrated that transcriptional activation function of Met was conserved between taxa. These findings may shed light on the relationship between functional diversification in hormonal signaling and the molecular evolution of hormone receptors.

Research Products

• [Int'l Joint Research] バーミンガム大学(英国)
• [Int'l Joint Research] インディアナ大学(米国)
• [Int'l Joint Research] NIVA(ノルウェー)
• [Journal Article] Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting (2016)
  • Author(s): Sumiya, Eri, Ogino, Yukiko, Toyota, Kenji, Miyakawa, Hitoshi, Miyagawa, Shinichi, Iguchi, Taisen
  • Journal Title: Journal of Applied Toxicology Volume: - Issue: 11 Pages: 1476-1485
  • DOI: 10.1002/jat.3306
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Diofenolan induces male offspring production through binding to juvenile hormone receptor in Daphnia magna (2015)
  • Author(s): Ryoko Abe, Kenji Toyota, Hitoshi Miyakawa, Haruna Watanabe, Tomohiro Oka, Shinichi Miyagawa, Hiroyo Nishide, Ikuo Uchiyama, Knut Erik Tollefsen, Taisen Iguchi, Norihisa Tatarazako
  • Journal Title: Aquatic Toxicology Volume: 159 Pages: 44-51
  • DOI: 10.1016/j.aquatox.2014.11.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ionotropic glutamate receptors mediate inducible defense in the water flea Daphnia pulex (2015)
  • Author(s): Miyakawa, H., Sato, T., Colbourne, J.K. and Iguchi, T.
  • Journal Title: PLoS ONE Volume: 10 Issue: 3 Pages: e0121324-e0121324
  • DOI: 10.1371/journal.pone.0121324
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex (2015)
  • Author(s): Kenji Toyota, Hitoshi Miyakawa, Katsushi Yamaguchi, Shuji Shigenobu, Yukiko Ogino, Norihisa Tatarazako, Shinichi Miyagawa, Taisen Iguchi
  • Journal Title: BMC Genomics Volume: 16 Issue: 1 Pages: 186-186
  • DOI: 10.1186/s12864-015-1392-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex (2015)
  • Author(s): Toyota K, Miyakawa H, Hiruta C, Furuta K, Ogino Y, Shinoda T, Tatarazako N, Miyagawa S, Shaw JR, Iguchi T
  • Journal Title: Journal of Insect Physiology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna. (2014)
  • Author(s): Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
  • Journal Title: Journal of Applied Toxicology Volume: 34 Issue: 5 Pages: 537-544
  • DOI: 10.1002/jat.2922
  • Peer Reviewed
• [Journal Article] Comparison of JH signaling in insects and crustaceans (2014)
  • Author(s): Hitoshi Miyakawa, Kenji Toyota, Eri Sumiya, Taisen Iguchi
  • Journal Title: Current Opinion in Insect Science Volume: 1 Pages: 81-87
  • DOI: 10.1016/j.cois.2014.04.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Roles of ecdysteroids for progression of reproductive cycle in the fresh water crustacean Daphnia magna (2014)
  • Author(s): Eri Sumiya, Yukiko Ogino, Hitoshi Miyakawa, Chizue Hiruta, Kenji Toyota, Shinichi Miyagawa, Taisen Iguchi
  • Journal Title: Frontiers in Zoology Volume: 11 Issue: 1 Pages: 60-60
  • DOI: 10.1186/s12983-014-0060-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 幼若ホルモン経路の進化をもたらす受容体型転写因子Methoprene-tolerantの新規制御関係獲得過程の解明 (2014)
  • Author(s): 宮川 一志, 井口 泰泉
  • Organizer: 第16回日本進化学会大会
  • Place of Presentation: 高槻現代劇場（大阪府高槻市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] Analysis of the reception and molecular signaling downstream of a crustacean juvenile hormone, methyl farnesoate, in Daphnia pulex (2014)
  • Author(s): Hitoshi Miyakawa, Masanao Sato, Yukiko Ogino, Shinichi Miyagawa, John K. Colbourne, Taisen Iguch
  • Organizer: 10th International Conference on Juvenile Hormones
  • Place of Presentation: 文部科学省研究交流センター（茨城県つくば市）
  • Year and Date: 2014-06-09 – 2014-06-13
• [Remarks] 宇都宮大学バイオサイエンス教育研究センター 環境生理学研究室
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/miyakawa/