| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Over the course of evolutionary time, juvenile hormone (JH) and its signaling pathway have acquired novel functions, both flexibly and repeatedly, while retaining their core roles related to the arthropod life cycle. To elucidate the process of functional diversification of JH, first, I compared the ligand selectivity of the JH receptor, Met, against four in four arthropod species (i.e., three insects: Drosophila, Aedes, Tribolium, and one crustacean: Daphnia) by performing a two-hybrid luciferase assay with Met and SRC orthologs. Obtained results suggest that the sequence structure of Met affects its ligand sensitivity. Next, I compared downstream signal cascade of Met between insect and crustacean species, and demonstrated that transcriptional activation function of Met was conserved between taxa. These findings may shed light on the relationship between functional diversification in hormonal signaling and the molecular evolution of hormone receptors.
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