Functional diversification process of juvenile hormone pathway through the acquisition of novel regulatory relationships
| Project/Area Number |
26840122
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Utsunomiya University (2015)
Okazaki Research Facilities, National Institutes of Natural Sciences (2014) |
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Principal Investigator |
Miyakawa Hitoshi 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (30631436)
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| Research Collaborator |
IGUCHI Taisen
MIYAGAWA Shinichi
OGINO Yukiko
HIRUTA Chizue
TOYOTA Kenji
SUMIYA Eri
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 幼若ホルモン / Methoprene-tolerant / 節足動物 / ミジンコ / リガンド特異性 |
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| Outline of Final Research Achievements |
Over the course of evolutionary time, juvenile hormone (JH) and its signaling pathway have acquired novel functions, both flexibly and repeatedly, while retaining their core roles related to the arthropod life cycle. To elucidate the process of functional diversification of JH, first, I compared the ligand selectivity of the JH receptor, Met, against four in four arthropod species (i.e., three insects: Drosophila, Aedes, Tribolium, and one crustacean: Daphnia) by performing a two-hybrid luciferase assay with Met and SRC orthologs. Obtained results suggest that the sequence structure of Met affects its ligand sensitivity. Next, I compared downstream signal cascade of Met between insect and crustacean species, and demonstrated that transcriptional activation function of Met was conserved between taxa. These findings may shed light on the relationship between functional diversification in hormonal signaling and the molecular evolution of hormone receptors.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting2016
Author(s)
Sumiya, Eri, Ogino, Yukiko, Toyota, Kenji, Miyakawa, Hitoshi, Miyagawa, Shinichi, Iguchi, Taisen
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Journal Title
Journal of Applied Toxicology
Volume: -
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Diofenolan induces male offspring production through binding to juvenile hormone receptor in Daphnia magna2015
Author(s)
Ryoko Abe, Kenji Toyota, Hitoshi Miyakawa, Haruna Watanabe, Tomohiro Oka, Shinichi Miyagawa, Hiroyo Nishide, Ikuo Uchiyama, Knut Erik Tollefsen, Taisen Iguchi, Norihisa Tatarazako
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Journal Title
Aquat Toxicol
Volume: 159
Pages: 44-51
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna.2014
Author(s)
Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
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Journal Title
Jounal of Applied Toxicology
Volume: 34
Pages: 537-544
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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