• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional diversification process of juvenile hormone pathway through the acquisition of novel regulatory relationships

Research Project

Project/Area Number 26840122
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionUtsunomiya University (2015)
Okazaki Research Facilities, National Institutes of Natural Sciences (2014)

Principal Investigator

Miyakawa Hitoshi  宇都宮大学, バイオサイエンス教育研究センター, 准教授 (30631436)

Research Collaborator IGUCHI Taisen  
MIYAGAWA Shinichi  
OGINO Yukiko  
HIRUTA Chizue  
TOYOTA Kenji  
SUMIYA Eri  
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords幼若ホルモン / Methoprene-tolerant / 節足動物 / ミジンコ / リガンド特異性
Outline of Final Research Achievements

Over the course of evolutionary time, juvenile hormone (JH) and its signaling pathway have acquired novel functions, both flexibly and repeatedly, while retaining their core roles related to the arthropod life cycle. To elucidate the process of functional diversification of JH, first, I compared the ligand selectivity of the JH receptor, Met, against four in four arthropod species (i.e., three insects: Drosophila, Aedes, Tribolium, and one crustacean: Daphnia) by performing a two-hybrid luciferase assay with Met and SRC orthologs. Obtained results suggest that the sequence structure of Met affects its ligand sensitivity. Next, I compared downstream signal cascade of Met between insect and crustacean species, and demonstrated that transcriptional activation function of Met was conserved between taxa. These findings may shed light on the relationship between functional diversification in hormonal signaling and the molecular evolution of hormone receptors.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 6 results,  Open Access: 4 results) Presentation (2 results) Remarks (1 results)

  • [Int'l Joint Research] バーミンガム大学(英国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] インディアナ大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] NIVA(ノルウェー)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting2016

    • Author(s)
      Sumiya, Eri, Ogino, Yukiko, Toyota, Kenji, Miyakawa, Hitoshi, Miyagawa, Shinichi, Iguchi, Taisen
    • Journal Title

      Journal of Applied Toxicology

      Volume: - Issue: 11 Pages: 1476-1485

    • DOI

      10.1002/jat.3306

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Diofenolan induces male offspring production through binding to juvenile hormone receptor in Daphnia magna2015

    • Author(s)
      Ryoko Abe, Kenji Toyota, Hitoshi Miyakawa, Haruna Watanabe, Tomohiro Oka, Shinichi Miyagawa, Hiroyo Nishide, Ikuo Uchiyama, Knut Erik Tollefsen, Taisen Iguchi, Norihisa Tatarazako
    • Journal Title

      Aquatic Toxicology

      Volume: 159 Pages: 44-51

    • DOI

      10.1016/j.aquatox.2014.11.015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ionotropic glutamate receptors mediate inducible defense in the water flea Daphnia pulex2015

    • Author(s)
      Miyakawa, H., Sato, T., Colbourne, J.K. and Iguchi, T.
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 3 Pages: e0121324-e0121324

    • DOI

      10.1371/journal.pone.0121324

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex2015

    • Author(s)
      Kenji Toyota, Hitoshi Miyakawa, Katsushi Yamaguchi, Shuji Shigenobu, Yukiko Ogino, Norihisa Tatarazako, Shinichi Miyagawa, Taisen Iguchi
    • Journal Title

      BMC Genomics

      Volume: 16 Issue: 1 Pages: 186-186

    • DOI

      10.1186/s12864-015-1392-9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex2015

    • Author(s)
      Toyota K, Miyakawa H, Hiruta C, Furuta K, Ogino Y, Shinoda T, Tatarazako N, Miyagawa S, Shaw JR, Iguchi T
    • Journal Title

      Journal of Insect Physiology

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna.2014

    • Author(s)
      Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
    • Journal Title

      Journal of Applied Toxicology

      Volume: 34 Issue: 5 Pages: 537-544

    • DOI

      10.1002/jat.2922

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of JH signaling in insects and crustaceans2014

    • Author(s)
      Hitoshi Miyakawa, Kenji Toyota, Eri Sumiya, Taisen Iguchi
    • Journal Title

      Current Opinion in Insect Science

      Volume: 1 Pages: 81-87

    • DOI

      10.1016/j.cois.2014.04.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Roles of ecdysteroids for progression of reproductive cycle in the fresh water crustacean Daphnia magna2014

    • Author(s)
      Eri Sumiya, Yukiko Ogino, Hitoshi Miyakawa, Chizue Hiruta, Kenji Toyota, Shinichi Miyagawa, Taisen Iguchi
    • Journal Title

      Frontiers in Zoology

      Volume: 11 Issue: 1 Pages: 60-60

    • DOI

      10.1186/s12983-014-0060-2

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 幼若ホルモン経路の進化をもたらす受容体型転写因子Methoprene-tolerantの新規制御関係獲得過程の解明2014

    • Author(s)
      宮川 一志, 井口 泰泉
    • Organizer
      第16回日本進化学会大会
    • Place of Presentation
      高槻現代劇場(大阪府高槻市)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of the reception and molecular signaling downstream of a crustacean juvenile hormone, methyl farnesoate, in Daphnia pulex2014

    • Author(s)
      Hitoshi Miyakawa, Masanao Sato, Yukiko Ogino, Shinichi Miyagawa, John K. Colbourne, Taisen Iguch
    • Organizer
      10th International Conference on Juvenile Hormones
    • Place of Presentation
      文部科学省研究交流センター(茨城県つくば市)
    • Year and Date
      2014-06-09 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Remarks] 宇都宮大学バイオサイエンス教育研究センター 環境生理学研究室

    • URL

      http://c-bio.mine.utsunomiya-u.ac.jp/miyakawa/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2022-01-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi