| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
It has been widely accepted that the glycolysis occurs only in the cytosol. In contrast to this view, eukaryotic species in Cercozoa and Stramenopila have been proposed to possess unique glycolytic pathway reside in mitochondria. To gain better understanding about the mitochondrial glycolytic pathway in cercozoans, glycolytic genes in representative species from major cercozoan subgroups were exhaustively searched. A combination of homology searches and phylogenetic analyses showed that glycolytic pathway expected to reside in mitochondria are limited to only a part of the diversity of Cercozoa. The result suggests that the mitochondrial glycolytic pathway is not an ancestral feature of the whole cercozoans but introduced into the lineage after the diversification. In the course of the project, an antibody for a mitochondrial glycolytic enzyme of an cercozoan species has been successfully produced and is expected to be useful to confirm the subcellular localization of the protein.
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