| Project/Area Number |
26840131
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Tokai University (2016)
Research Institute for Humanity and Nature (2014-2015) |
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Principal Investigator |
Muto Nozomu 東海大学, 生物学部, 講師 (50724267)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIKAWA Satoshi 総合地球環境学研究所, 教授 (40433908)
MOTOMURA Hiroyuki 鹿児島大学, 総合研究博物館, 教授 (90433086)
TAKESHIMA Hirohiko 総合地球環境学研究所, 特任助教 (50573086)
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| Research Collaborator |
MUTO Fumihito 東海大学, 海洋学部, 准教授
Babaran Ricardo P. フィリピン大学, 教授
KAKIOKA Ryo 国立遺伝学研究所, 研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 南シナ海 / 更新世 / 系統地理 / 魚類 / ミトコンドリアDNA / マイクロサテライト / 種分化 / 二次的接触 |
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| Outline of Final Research Achievements |
We aimed to clarify historical processes contributing to the marine fish diversity in the South China Sea, focusing on the Pleistocene glacial-interglacial cycles. Mitochondrial DNA sequencing was employed to infer historical population dynamics in 18 selected fish species. In some species, specimens from the South China Sea were separated into two divergent clades on mtDNA gene tree, suggesting that they have experienced historical divergence due to the sea level changes in the Pleistocene, followed by secondary contact.
For a subset of the aforementioned species, nuclear DNA divergence was tested using microsatellite genotyping. The species tested exhibited clear nuclear divergence between the two mtDNA clades, indicating that they are representing separate species. Taken together, our results showed that the Pleistocene glacial-interglacial cycles have contributed to the present marine fish diversity in the South China Sea, both at the intraspecific- and the species-level.
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