Historical biogeography of fishes of the South China Sea in relation to the Pleistocene glacial-interglacial cycles

• Project/Area Number: 26840131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Tokai University (2016); Research Institute for Humanity and Nature (2014-2015)
• Principal Investigator: Muto Nozomu 東海大学, 生物学部, 講師 (50724267)
• Co-Investigator(Renkei-kenkyūsha): ISHIKAWA Satoshi 総合地球環境学研究所, 教授 (40433908) ; MOTOMURA Hiroyuki 鹿児島大学, 総合研究博物館, 教授 (90433086) ; TAKESHIMA Hirohiko 総合地球環境学研究所, 特任助教 (50573086)
• Research Collaborator: MUTO Fumihito 東海大学, 海洋学部, 准教授 ; Babaran Ricardo P. フィリピン大学, 教授 ; KAKIOKA Ryo 国立遺伝学研究所, 研究員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 南シナ海 / 更新世 / 系統地理 / 魚類 / ミトコンドリアDNA / マイクロサテライト / 種分化 / 二次的接触

Outline of Final Research Achievements

We aimed to clarify historical processes contributing to the marine fish diversity in the South China Sea, focusing on the Pleistocene glacial-interglacial cycles. Mitochondrial DNA sequencing was employed to infer historical population dynamics in 18 selected fish species. In some species, specimens from the South China Sea were separated into two divergent clades on mtDNA gene tree, suggesting that they have experienced historical divergence due to the sea level changes in the Pleistocene, followed by secondary contact.
For a subset of the aforementioned species, nuclear DNA divergence was tested using microsatellite genotyping. The species tested exhibited clear nuclear divergence between the two mtDNA clades, indicating that they are representing separate species. Taken together, our results showed that the Pleistocene glacial-interglacial cycles have contributed to the present marine fish diversity in the South China Sea, both at the intraspecific- and the species-level.

Research Products

• [Int'l Joint Research] フィリピン大学ビサヤス校(フィリピン)
• [Int'l Joint Research] ベトナム海洋環境・資源研究 所(ベトナム)
• [Int'l Joint Research] タイ国立博物館(タイ)
• [Int'l Joint Research] トレンガヌ大学(マレーシア)
• [Int'l Joint Research] 国立海洋生物博物館(台湾)
• [Int'l Joint Research] University of the Philippines Visayas(フィリピン)
• [Int'l Joint Research] National Science Museum/SEAFDEC(タイ)
• [Int'l Joint Research] Institute of Mar. Environ. Resour.(ベトナム)
• [Int'l Joint Research] Universiti Malaysia Terengganu/Universiti Putra Malaysia(マレーシア)
• [Journal Article] Rapid and effective isolation of candidate sequences for development of microsatellite markers in 30 fish species by using kit-based target capture and multiplexed parallel sequencing (2017)
  • Author(s): Hirohiko Takeshima, Nozomu Muto, Yasuyuki Sakai, Naoya Ishiguro, Keiichiro Iguchi, Satoshi Ishikawa, Mutsumi Nishida
  • Journal Title: Conservation Genetics Resources Volume: online first Issue: 3 Pages: 1-12
  • DOI: 10.1007/s12686-017-0699-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A new species of labrid fish Oxycheilinus samurai from the western Pacific Ocean. (2017)
  • Author(s): Fukui Y, Muto N, Motomura H.
  • Journal Title: Ichthyological Research Volume: 未定 Issue: 2 Pages: 212-220
  • DOI: 10.1007/s10228-016-0561-4
  • NAID: 40021169708
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rapid and cost-effective molecular identification of the three mackerel species of the genus Rastrelliger (Perciformes: Scombridae) using PCR-RFLP analysis. (2017)
  • Author(s): Muto N, Takeshima H, Kakioka R, Alama UB, Guzman AMT, Cruz RS, Gaje AC, Traifalgar RFM, Motomura H, Muto F, Babaran RP, Ishikawa S.
  • Journal Title: Marine Biodiversity Volume: 未定 Issue: 2 Pages: 609-611
  • DOI: 10.1007/s12526-016-0537-7
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Stable isotope analysis suggests the existence of multiple populations of streaked spinefoot (Siganus javus L.) in Bandon Bay, Southern Thailand. (2016)
  • Author(s): Okamoto Y, Muto N, Kon K, Watanabe K, Yoshikawa T, Salaenoi J, Ishikawa S.
  • Journal Title: International Aquatic Research Volume: 8 Pages: 169-178
  • NAID: 120006346266
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genetic and morphological differences among the three species of the genus Rastrelliger (Perciformes: Scombridae). (2016)
  • Author(s): Muto N, Alama UB, Hata H, Guzman AMT, Cruz R, Gaje A, Traifalgar RFM, Kakioka R, Takeshima H, Motomura H, Muto F, Babaran RP, Ishikawa S.
  • Journal Title: Ichthyological Research Volume: 63 Pages: 275-287
  • NAID: 40020815940
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genetic and morphological differences among the three species of the genus Rastrelliger (Perciformes: Scombridae). (2016)
  • Author(s): Nozomu Muto, Ulysses B. Alama, Harutaka Hata, Armi May T. Guzman, Ramon Cruz, Arnold Gaje, Rex Ferdinand M. Traifalgar, Ryo Kakioka, Hirohiko Takeshima, Hiroyuki Motomura, Fumihito Muto, Ricardo P. Babaran, Satoshi Ishikawa
  • Journal Title: Ichthyological Research Volume: 63 Issue: 2 Pages: 275-287
  • DOI: 10.1007/s10228-015-0498-z
  • NAID: 40020815940
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Solving the mixed-stock problem by an integrative approach: a case study of the genus Rastrelliger (Pisces: Perciformes: Scombridae). (2016)
  • Author(s): Muto N, Alama UB, Guzman AMT, Cruz SR, Gaje CA, Kakioka R, Takeshima H, Hata H, Motomura H, Muto F, Traifalgar RFM, Babaran RP, Ishikawa S.
  • Organizer: The 25th Federation of Asian and Oceanian Biochemists and Molecular Biologists Conference
  • Place of Presentation: Philippine International Convention Center
  • Year and Date: 2016-12-05
  • Int'l Joint Research
• [Presentation] 西太平洋から得られたベラ科ホホスジモチノウオ属の2未記載種 (2016)
  • Author(s): 福井美乃，武藤望生，本村浩之
  • Organizer: 2016年度日本魚類学会年会
  • Place of Presentation: 岐阜大学
  • Year and Date: 2016-09-23
• [Presentation] 西太平洋におけるオニアジの隠蔽種と交雑 (2016)
  • Author(s): 武藤望生，Arnold GAJE, Ramon CRUZ，Ulysses ALAMA，Armi GUZMAN，Rex TRAIFALGAR，Ricardo BABARAN，本村浩之，武藤文人，武島弘彦，石川智士
  • Organizer: 2016年度日本魚類学会年会
  • Place of Presentation: 岐阜大学
  • Year and Date: 2016-09-23
• [Presentation] Highly divergent lineages in the freshwater needlefish Xenentodon within the Mekong River System. (2016)
  • Author(s): Kakioka R, Muto N, Kounthongbang A, Ito S, Hort S, Takeshima H, Ishikawa S, Iguchi K.
  • Organizer: Annual meeting of the Ichthyological Society of Taiwan and the Asian Society of Ichythyologists
  • Place of Presentation: Nangang Exhibition Center, Taiwan
  • Year and Date: 2016-05-18
  • Int'l Joint Research
• [Presentation] Fish species diversity around Bidong Island off Terengganu, Peninsular Malaysia (2016)
  • Author(s): Koeda K, Ghaffar MA, Arshad A, Giat SY, Kadir STSA, Ramasamy AMA, Ibrahim YS, Nakae M, Muto N, Yoshiura A, Matsuo R, Kimura S, Motomura H.
  • Organizer: Asian CORE-COMSEA Seminar on Coastal Ecosystems in Southeast Asia
  • Place of Presentation: ATMOSPHERE AND OCEAN RESEARCH INSTITUTE, THE UNIVERSITY OF TOKYO
  • Year and Date: 2016-02-24
  • Int'l Joint Research
• [Presentation] Reef and shore fishes of Ha Long Bay, a World Heritage Site in northern Vietnam (2016)
  • Author(s): Kimura S, Quan NV, Motomura H, Nakae M, Imamura H, Muto N, The ND, Chien PV, Yoshida T, Hibino Y, Jeong B, Suzuki H, Tashiro S, Matsuura K.
  • Organizer: Asian CORE-COMSEA Seminar on Coastal Ecosystems in Southeast Asia
  • Place of Presentation: ATMOSPHERE AND OCEAN RESEARCH INSTITUTE, THE UNIVERSITY OF TOKYO
  • Year and Date: 2016-02-24
  • Int'l Joint Research
• [Presentation] Genetic and morphological differences among the three species of the genus Rastrelliger (Perciformes: Scombridae) and their identification using PCR-RFLP. (2016)
  • Author(s): Muto N, Alama UB, Guzman AMT, Cruz R, Gaje A, Kakioka R, Takeshima H, Hata H, Motomura H, Muto F, Traifalgar RFM, Babaran RP, Ishikawa S
  • Organizer: International Conference on Fisheries and Aquatic Sciences.
  • Place of Presentation: Amigo Terrace Hotel
  • Year and Date: 2016-01-26
  • Int'l Joint Research
• [Presentation] Coastal fish diversity in the South China Sea. International Workshop on Area-capability studies in Coastal Zone of Southeast Asia. (2015)
  • Author(s): Muto N, Kakioka R, Takeshima H, Alama UB, Hata H, Guzman AMT, Cruz R, Gaje A, Traifalgar RFM, Yangphonkhan B, Phuttarakusa K, Arnupapboon S, Vilasri V, Babaran RP, Motomura H, Muto F.
  • Organizer: International Workshop on Area-capability studies in Coastal Zone of Southeast Asia.
  • Place of Presentation: Research Institute for Humanity and Nature
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Spatiotemporal variations in δ13C and δ15N values of demersal fishes captured by small scale fisheries in Bandon Bay, Southern Thailand (2015)
  • Author(s): Yuki Okamoto, Nozomu Muto, Koetsu Kon, Kazuya Watanabe, Takashi Yoshikawa Jintana Salaenoi, Satoshi Ishikawa.
  • Organizer: 平成27年度日本水産学会秋季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2015-09-23
• [Presentation] グルクマ属魚類3種の遺伝的・形態的差異 (2015)
  • Author(s): 武藤望生，Ulysses ALAMA，畑 晴陵，武島弘彦，柿岡 諒，Arnold GAJE， Ramon CRUZ，Armi GUZMAN，Rex TRAIFALGAR，Ricardo BABARAN，本村浩之，武藤文人，石川智士．
  • Organizer: 2015年度日本魚類学会年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2015-09-09
• [Presentation] 南シナ海におけるメガネタマガシラの遺伝的集団構造 (2015)
  • Author(s): 柿岡諒，武藤望生，武島弘彦，Osman MUDA，Sukchai ARNUPAPBOON，Kamolrat PHUTTHARAKSA，Nguyen VAN QUAN, Arnold GAJE，Ramon CRUZ，Ulysses ALAMA，Armi GUZMAN，Rex TRAIFALGAR，Ricardo BABARAN，本村浩之，武藤文人，石川智士．
  • Organizer: 2015年度日本魚類学会年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2015-09-09
• [Presentation] Genetic population structure of commercially important coastal fishes in the South China Sea (2014)
  • Author(s): Nozomu Muto
  • Organizer: Mini-symposium on marine fish biodiversity in Southeast Asia
  • Place of Presentation: Institute of Marine Environment and Resources, Vietnam Academy of Science and Technology
  • Year and Date: 2014-12-10
  • Invited
• [Presentation] 南シナ海におけるアジ科魚類3種の遺伝的集団構造 (2014)
  • Author(s): 武藤望生，柿岡 諒，Osman Bin MUDA，Sukchai ARNUPAPBOON，Kamolrat PHUTTHARAKSA，Arnold GAJE, Ramon CRUZ, Ulysses B. ALAMA, Rex Ferdinand M. TRAIFALGAR, Ricardo P. BABARAN，武島弘彦，本村浩之，武藤文人，石川智士
  • Organizer: 2014年度日本魚類学会年会
  • Place of Presentation: 神奈川県立 生命の星・地球博物館
  • Year and Date: 2014-11-14 – 2014-11-17
• [Book] Commercial and Bycatch Market Fishes of Panay Island, Republic of the Philippines. (2017)
  • Author(s): Motomura H, Alama UB, Muto N, Babaran RP, Ishikawa S
  • Total Pages: 246
  • Publisher: The Kagoshima University Museum, Kagoshima, University of the Philippines Visayas, Iloilo, and Research Institute for Humanity and Nature, Kyoto
• [Book] Coastal Area Capability Enhancement in Southeast Asia Project Interim Report (2015)
  • Author(s): Coastal Area Capability Enhancement in Southeast Asia” Project (ed)
  • Total Pages: 554
  • Publisher: Coastal Area Capability Enhancement in Southeast Asia” Project