| Project/Area Number |
26840152
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Ryukoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生物量 / 代謝量 / 環境核酸 / 環境評価 / 測定精度 / 遺伝子 / 水棲生物 |
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| Outline of Final Research Achievements |
In this study, we tried to improve the accuracy of the quantification of environmental nucleic acids (environmental DNA and environmental RNA) to expand the application of eDNA and eRNA analyses for the estimation of biomass and metabolic rate of aquatic organisms. Environmental DNA has known to degrade rapidly in water, and it causes problem in the quantification of eDNA. We developed a new on site filtration system. Immediate filtration followed by freezing of the obtained filter sample provided eDNA samples which precisely reflected the real concentration of eDNA at the sampling time. On the other hand, the quantification accuracy of eRNA did not improved well due to an unexpected large variation of measurement values. However, we succeeded in the species-specific detection of mRNA relating to the metabolism of common carp from field water sample, and found that eRNA might remain in water at quantifiable concentration for at least 24 hours by a tank experiment.
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