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DNA barcording analysis on oral mircorbiome in ancient dental calculus Japanese skeltal remain

Research Project

Project/Area Number 26840158
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Physical anthropology
Research InstitutionAsahi University

Principal Investigator

Yano Wataru  朝日大学, 歯学部, 助教 (80600113)

Research Collaborator Amanda Henry G.  マックスプランク進化人類学研究所
IMAI Hiroo   (60314176)
HAYAKAWA Takashi  京都大学, 霊長類研究所, 特定助教 (00758493)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords口腔細菌叢 / 次世代シーケンサー / DNAメタバーコーディング解析 / 縄文人 / 歯石 / 歯周病 / 霊長類 / 口腔細菌 / 古人骨 / 古病理学 / 人類学 / 縄文人骨 / 歯垢 / 口腔内細菌叢 / 次世代アンプリコンシーケンス解析 / 古代人骨 / メタゲノム解析
Outline of Final Research Achievements

In the planned research, I successfully analyzed oral microbiome of ancient human population of Japan (Jomon people) using DNA meta-barcording technique with Next generation sequencer (MiSeq, Illumina). I used ancient remain of dental calculus from Jomon people in Kyoto University. The material contained representative oral pathogenic species such as porphyromonas spp. which indicates the possible infection of periodontal diseases. In addition to the human data, to explore the diversity of pathogenic oral microbial species of closely related primates species such as Chimpanzees (Pan troglodytes) and Lesser slow loris (Nycticebus pygmaeus) and found new species of oral pathogenic species.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] 歯の人類学分科会 平成26年度シンポジウム「歯の付着物をめぐって」2015

    • Author(s)
      金澤 英作, 小山田 常一, 北川 賀一, 真鍋 義孝, 矢野 航, HENRY Amanda G., 今井 啓雄, 江㞍 貞一, 山崎 京美, 高橋 正志, 遠藤 秀紀, 米田 穣, 覚張 隆史, 金子 浩昌
    • Journal Title

      Anthropological Science (Japanese Series)

      Volume: 123 Issue: 1 Pages: 51-58

    • DOI

      10.1537/asj.123.51

    • NAID

      130005078078

    • ISSN
      1344-3992, 1348-8813
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒト上科霊長類における歯周病2016

    • Author(s)
      矢野航、橋本千絵、清水大輔
    • Organizer
      第60 回プリマーテス研究会
    • Place of Presentation
      日本モンキーセンター
    • Year and Date
      2016-01-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 古人骨歯石内に残る微化石解析2014

    • Author(s)
      矢野航、Henry G. Amanda、今井啓雄、江㞍貞一
    • Organizer
      第68回日本人類学会
    • Place of Presentation
      浜松
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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