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Identification of the MdMYB110a binding motif and the endogenous factors in the coloration of type 2 red-fleshed apples

Research Project

Project/Area Number 26850015
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionNagoya University

Principal Investigator

OTAGAKI Shungo  名古屋大学, 生命農学研究科, 助教 (50597789)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords赤果肉リンゴ / MYB転写因子 / 植物ホルモン / エチレン / アブシシン酸 / リンゴ / 赤果肉 / 転写因子 / MYB / 遺伝子発現 / 果実発育・成熟 / ウイルスベクター / プロモーター解析 / 園芸学 / プロモーター機能解析
Outline of Final Research Achievements

In this study, I aimed to identify the MdMYB110a binding motif and to clarify the contribution of plant hormones in the coloration of type 2 red-fleshed apples. For the first research topic, I performed the ectopic expression of MdMYB110a protein for chromatin immunoprecipitation analysis and the reporter assay using the agroinfiltration system but failed to identify the binding motif. For the second research topic, I revealed that the coloration of type 2 red-fleshed apples is not induced by ethylene, which is known as a major plant hormone accumulating during the apple fruit maturation, but by ABA.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2017 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Varietal differences in phenolic compounds metabolism of type 2 red-fleshed apples.2017

    • Author(s)
      H. Sato, S. Otagaki, P. Saelai, S. Kondo, K. Shiratake, S. Matsumoto
    • Journal Title

      Scientia Hortculturae

      Volume: 219 Pages: 1-9

    • DOI

      10.1016/j.scienta.2017.02.041

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Breeding depression of red flesh apple progenies containing both functional MdMYB10 and MYB110a_JP genes.2015

    • Author(s)
      Y. Hamada, H. Sato, S. Otagaki, K. Okada, K. Abe and S. Matsumoto
    • Journal Title

      Plant Breeding

      Volume: 134 Issue: 2 Pages: 239-246

    • DOI

      10.1111/pbr.12255

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Ⅱ型赤果肉リンゴにおける着色程度と二次代謝物蓄積様式の関係2017

    • Author(s)
      太田垣駿吾、佐藤秀人、サエライ パリチャット、近藤悟、白武勝裕、松本省吾
    • Organizer
      園芸学会平成29年度春季大会
    • Place of Presentation
      日本大学生物資源科学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] リンゴ果皮・果肉着色に関わるMBW転写因子群の特製とマーカー育種2015

    • Author(s)
      小野裕馬、佐藤秀人、前島勤、太田垣駿吾、白武勝裕、松本省吾
    • Organizer
      平成27年度園芸学会秋季大会
    • Place of Presentation
      徳島大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] タイプ2赤果肉リンゴ品種・系統ごとの着色関連遺伝子群の発現パターン2014

    • Author(s)
      佐藤秀人、太田垣駿吾ら
    • Organizer
      平成26年度園芸学会秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2023-03-23  

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