Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Outline of Final Research Achievements |
Virus-induced gene silencing (VIGS) has been used as a rapid and effective tool for functional analysis of genes in various plants. In this study, we assessed the VIGS efficiency of Apple latent spherical virus (ALSV) vectors in a wide range of Prunus species and cultivars. We found that ALSV vectors could successfully induce VIGS of endogenous PHYTOENE DESATURASE (PDS) genes in several cultivars of apricot, sweet cherry, and almond, although ALSV infectivity and VIGS efficiency seemed to vary depending on species and/or cultivar in Prunus. Further optimization of viral inoculation procedures and growth conditions for infected plants will lead to the full use of ALSV vectors for evaluation of gene functions or various practical studies in Prunus.
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