| Project/Area Number |
26850019
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | プロアントシアニジン / カキ / 没食子酸 / ガレート / 果実 / シキミ産生合成 / 生合成 |
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| Outline of Final Research Achievements |
In order to characterize DkDHD/SDH and DkSCPL2, which is supposed to be involved in proanthocyanidin(PA) accumulation in persimmon fruit, we tried transformation of persimmon and tabacco with these genes using Agrobacterium tumefaciens. The PAs in persimmon callus overexpressed DkSCPL2 showed higher amount, however, the ratio of flavan 3-ols-gallates were not altered. The DkDHD/SDHs showed activity in shikimic acid pathway to produce final product of phenylalanine as the precursor for flavonoid biosynthetic pathway. The activity as gallic acid biosynthesis, however, was not detected in our assay. Therefore, the other enzyme, but not DHD/SDH, for dehydroshikimate dehydrogenase should be involved in gallic acid biosynthesis.
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