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Functional analysis of flowering-related gene in over-wintering buds of gentian

Research Project

Project/Area Number 26850020
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionTokyo University of Science

Principal Investigator

Imamura Tomohiro  東京理科大学, 基礎工学部, 研究員 (20468705)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords休眠 / 越冬 / 多年生植物 / リンドウ / Flowering Locus T / 越冬組織 / SOC1-like / FLC
Outline of Final Research Achievements

Gentian is a perennial plant and known as ornamental flowers. To reveal the mechanism of overwintering buds formation, we first evaluated the movement of GtFT1 protein from aerial- to underground- organ in gentian. However, we could not find the GtFT1 protein movement. Next, we performed expression analysis of overwintering buds. GtSOC1L was highly expressed in overwintering buds, whereas GtFLC was broadly expressed in overwintering buds. Moreover, Transgenic gentian plantlets expressing GtSOC1L showed excessive formation of overwintering buds compared to wild type. These results implied that the GtSOC1L play important role in the formation of overwintering buds in gentian.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (1 results)

  • [Journal Article] Production of recombinant thanatin in watery rice seeds that lack an accumulation of storage starch and proteins2016

    • Author(s)
      Imamura T, Sekine KT, Yamashita T, Kusano H, and Shimada H.
    • Journal Title

      Journal of Biotechnology

      Volume: 219 Pages: 28-33

    • DOI

      10.1016/j.jbiotec.2015.12.006

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of spermidine synthase and spermine synthase - the polyamine-synthetic enzymes that induce early flowering in Gentiana triflora.2015

    • Author(s)
      Imamura T, Fugita K, Tasaki K, Higuchi A, and Takahashi H
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 463 Issue: 4 Pages: 781-786

    • DOI

      10.1016/j.bbrc.2015.06.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] High concentrations of sucrose induce overwintering bud formation in gentian plantlets cultured in vitro2014

    • Author(s)
      Tomohiro Imamura, Atsumi Higuchi, Ken-Taro Sekine, Tetsuro Yamashita, Hideyuki Takahashi
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 2 Pages: 97-104

    • DOI

      10.5511/plantbiotechnology.13.1211a

    • NAID

      130003391370

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The gentio- oligosaccharide gentiobiose functions in the modulation of bud dormancy in the herbaceous perennial Gentiana2014

    • Author(s)
      高橋秀行、今村智弘、金野尚武、竹田匠、藤田晃平、小西照子、西原昌宏、内宮博文
    • Journal Title

      Plant Cell

      Volume: 26 Issue: 10 Pages: 3949-3963

    • DOI

      10.1105/tpc.114.131631

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] リンドウの光周性花成経路に関わるGtCO遺伝子の探索と機能解析2014

    • Author(s)
      今村智弘、樋口敦美、高橋秀行
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会
    • Place of Presentation
      アイーナ(いわて県民情報交流センター)(岩手県)
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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