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Elucidation of xanthone biosynthesis in Gentiana.

Research Project

Project/Area Number 26850021
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionIwate Biotechnology Research Center

Principal Investigator

Sasaki Nobuhiro  公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (80422088)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsキサントン / リンドウ / 配糖化酵素 / フラボノイド
Outline of Final Research Achievements

The RNA-seq analysis using the RNAs extracted from the leaves of Gentiana scabra. We use this and EST database of G. triflora constructed before to pick up the genes involved in xanthon and flavonoid biosynthesis. Twenty three (G. scabra) and 6 (G. triflora) of UDP-glucose dependent glucosyltransferase (UGT) homologous contigs including the putative first ATG sequence was found by BLAST search. The full lengths of their coding region were isolated and the recombinant proteins were produced using the E. coli expression system. One of them isolated from G. triflora showed UGT activity toward flavones. NMR and MS analysis showed that the compound produced by the reaction was the flavone linked to glucose by C-C bond. Eight recombinant protein produced by the cDNAs isolated from G. scabra showed UGT activities toward norathyriol, an aglycone of xanthones. They also showed the activities toward other flavonoids and produced multiple reaction products.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora2015

    • Author(s)
      Sasaki, N., Nishizaki, Y., Yamada, E., Tatsuzawa, F., Nakatsuka, T. Takahashi, H., Nishihara, M.
    • Journal Title

      FEBS Letters

      Volume: 589 Issue: 1 Pages: 182-187

    • DOI

      10.1016/j.febslet.2014.11.045

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 日本のリンドウ園芸品種に含まれるポリフェノール類の解析2014

    • Author(s)
      佐々木伸大、山田恵理、西﨑雄三、中塚貴司、立澤文見、樋口敦美、藤田晃平、高橋秀行、西原昌宏
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] リンドウからのフラボンC−配糖化酵素遺伝子の単離2014

    • Author(s)
      佐々木伸大、山田恵理、西﨑雄三、中塚貴司、立澤文見、樋口敦美、藤田晃平、高橋秀行、西原昌宏
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      盛岡
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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