Research Project
Grant-in-Aid for Young Scientists (B)
The RNA-seq analysis using the RNAs extracted from the leaves of Gentiana scabra. We use this and EST database of G. triflora constructed before to pick up the genes involved in xanthon and flavonoid biosynthesis. Twenty three (G. scabra) and 6 (G. triflora) of UDP-glucose dependent glucosyltransferase (UGT) homologous contigs including the putative first ATG sequence was found by BLAST search. The full lengths of their coding region were isolated and the recombinant proteins were produced using the E. coli expression system. One of them isolated from G. triflora showed UGT activity toward flavones. NMR and MS analysis showed that the compound produced by the reaction was the flavone linked to glucose by C-C bond. Eight recombinant protein produced by the cDNAs isolated from G. scabra showed UGT activities toward norathyriol, an aglycone of xanthones. They also showed the activities toward other flavonoids and produced multiple reaction products.
All 2015 2014
All Journal Article (1 results) (of which Peer Reviewed: 1 results, Acknowledgement Compliant: 1 results) Presentation (2 results)
FEBS Letters
Volume: 589 Issue: 1 Pages: 182-187
10.1016/j.febslet.2014.11.045
