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Construction of the information infrastructure for genomic research and phytochrome gene mutation analysis in fig (Ficus carica L.)

Research Project

Project/Area Number 26850025
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionFukuoka Agricultural and Forestry Research Center

Principal Investigator

Ikegami Hidetoshi  福岡県農林業総合試験場, 豊前分場, 研究員 (40502414)

Co-Investigator(Renkei-kenkyūsha) KUHARA Satoru  九州大学, 農学研究院, 教授 (00153320)
TASHIRO Kosuke  九州大学, 農学研究院, 准教授 (00192170)
SHIRASAWA Kenta  公益財団法人かずさDNA研究所, 先端研究部, 主任研究員 (60527026)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsドラフトゲノム解読 / 遺伝子制御 / 形質転換 / 花成 / GWAS / RNA-Seq / 相関解析 / ネットワーク解析 / Dof転写因子 / 高密度連鎖地図 / イチジク / ドラフトゲノム / 解読 / 次世代シークエンサー
Outline of Final Research Achievements

The purpose of this study is to construct a genome information base and uncover the background of the expression control of the <i>FcFT1</i> gene related to flower bud differentiation characteristic to fig (<i>Ficus carica</i> L.).
The first draft genome assembly was establised and high density linkage map were constructed with NGS technologies. Next, based on the large-scale transcriptome profile, plural candidate genes for <i>FcFT1</i> regulation were identified. Simultaneously, A. thaliana transformants with a candidate gene were created and those transfomants were evaluated.
From the above, a part of <i>FcFT1</i> expression control was revealed. At the same time, the genome information infrastructure has been prepared, making it possible to efficiently analyze and evaluate genomic regions and genes involved in various traits such as flowering.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2017 2015 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.).2017

    • Author(s)
      Mori K, Shirasawa K, Nogata H, Hirata C, Tashiro K, Habu T, Kim S, Himeno S, Kuhara S, Ikegami H
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 41124-41124

    • DOI

      10.1038/srep41124

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] RAD-seqによるイチジク連鎖地図の作成2015

    • Author(s)
      池上秀利・白澤健太・森一樹・田代康介・久原哲・羽生剛・平田千春・姫野修一
    • Organizer
      園芸学会平成27年度秋季大会
    • Place of Presentation
      徳島大学常三島キャンパス
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Remarks] 世界で初めてイチジクのゲノム配列の解読に成功

    • URL

      http://www.pref.fukuoka.lg.jp/press-release/itizikugenomu.html

    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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