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Characterization of CERK1 interacting protein and CERK1 intracellular regulation

Research Project

Project/Area Number 26850028
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant protection science
Research InstitutionMeiji University

Principal Investigator

Desaki Yoshitake  明治大学, 農学部, 助教 (80711647)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords植物免疫 / シロイヌナズナ / キチン / MAMP / CERK1 / ユビキチン / PAMP
Outline of Final Research Achievements

Chitin is a major component of fungal cell wall and induces various defense responses in plants. After the perception of chitin by the cell surface receptor, chitin-induced immune signaling is activated through the receptor-interacting proteins. In this study, it was revealed that a novel receptor interacting protein, which was identified by this researcher, contributes to chitin-induced defense responses. In addition, this interacting protein was phosphorylated by the CERK1 kinase domain. This phosphorylation was suggested to play a role in activating the interacting protein.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (28 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (23 results) (of which Int'l Joint Research: 5 results) Book (1 results)

  • [Journal Article] The structure of the lipooligosaccharide from Xanthomonas oryzae pv. oryzae the causal agent of the bacterial leaf blight in rice2016

    • Author(s)
      F. Di Lorenzo, A. Palmigiano, A. Silipo, Y. Desaki, D. Garozzo, R. Lanzetta, N. Shibuya, A. Molinaro.
    • Journal Title

      Carbohydrate Research

      Volume: 427 Pages: 38-43

    • DOI

      10.1016/j.carres.2016.03.026

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Modulation of plant defense responses to herbivores by simultaneous recognition of different herbivore-associated elicitors in rice2016

    • Author(s)
      T. Shinya, Y. Hojo, Y. Desaki, J.T. Christeller, K. Okada, N. Shibuya, I. Galis.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 32537-32537

    • DOI

      10.1038/srep32537

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autophosphorylation of specific threonine and tyrosine residues in Arabidopsis CERK1 is essential for the activation of chitin-induced immune signaling2016

    • Author(s)
      M. Suzuki, M. Shibuya, H. Shimada, N. Motoyama, M. Nakashima, S. Takahashi, K. Suto, I. Yoshida, S. Matsui, N. Tsujimoto, M. Ohnishi, Y. Ishibashi, Z. Fujimoto, Y. Desaki, H. Kaku, K. Kito, N. Shibuya
    • Journal Title

      Plant Cell Physiol.

      Volume: 51 Issue: 11 Pages: 2312-2322

    • DOI

      10.1093/pcp/pcw150

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Quantification of stimulus-induced callose spots on plant materials2016

    • Author(s)
      M. Kohari, K. Yashima, Y. Desaki, N. Shibuya.
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 1 Pages: 11-17

    • DOI

      10.5511/plantbiotechnology.15.1120a

    • NAID

      130005146720

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ユビキチンリガーゼPUB4はCERK1によるリン酸化を介してシグナル伝達を制御する2017

    • Author(s)
      小泉春樹、三浦駿希、小針政輝、鈴木丸陽、澤進一郎、石橋裕子、紀藤圭治、出崎能丈、渋谷直人、賀来華江
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナCERK1の自己リン酸化部位Y428はキナーゼの活性化を通じてキチン応答を制御する2017

    • Author(s)
      鈴木丸陽、渡邉巧、出崎能丈、渋谷直人、賀来華江
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 可溶性ペプチドグリカン断片の調製とそのエリシター活性評価2016

    • Author(s)
      岩瀬良介、島田日加瑠、出崎能丈、賀来華江、渋谷直人
    • Organizer
      平成28年度植物感染生理談話会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2016-08-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナCERK1のユビキチン化部位の同定と機能解析2016

    • Author(s)
      松井紗樹、中島正登、三浦駿希、田中優太、大西美帆子、紀藤圭治、出崎能丈、賀来華江、渋谷直人
    • Organizer
      平成28年度植物感染生理談話会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2016-08-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] キチン受容体キナーゼCERK1の自己リン酸化による制御機構の解析2016

    • Author(s)
      吉田一誠、鈴木丸陽、須藤健吉、渋谷匡俊、島田日加瑠、元山記子、高橋昌平、大西美帆子、石橋裕子、出崎能丈、賀来華江、紀藤圭治、渋谷直人
    • Organizer
      平成28年度植物感染生理談話会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2016-08-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] イネキチン受容体の動態解析へ向けた実験系構築2016

    • Author(s)
      増田善樹、栗原渉、渡邊瞳、関口吉則、早船真広、出崎能丈、西澤洋子、澁谷直人、賀来華江
    • Organizer
      平成28年度植物感染生理談話会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2016-08-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] An E3 ubiquitin ligase, PUB4, regulates immune signaling through the interaction with Arabidopsis CERK12016

    • Author(s)
      Y. Desaki, S. Takahashi, H. Koizumi, T. Miura, K. Yashima, Y. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, H. Kaku, N. Shibuya。
    • Organizer
      IS-MPMI XVII congress
    • Place of Presentation
      Portland, U.S.A.
    • Year and Date
      2016-07-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and functional analysis of autophosphorylation sites in Arabidopsis CERK12016

    • Author(s)
      M. Suzuki, K. Suto, M. Shibuya, H. Shimada, N. Motoyama, S. Takahashi, I Yoshida, M. Ohnishi, Y. Ishibashi, Z. Fujimoto, Y. Desaki, H. Kaku, K. Kito, N. Shibuya.
    • Organizer
      IS-MPMI XVII congress
    • Place of Presentation
      Portland, U.S.A.
    • Year and Date
      2016-07-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Plant defense responses to herbivores involve recognition of independent herbivore-associated molecular patterns in rice2016

    • Author(s)
      T. Shinya, Y. Hojo, Y. Desaki, J. T. Christeller, K. Okada, N. Shibuya, I. Galis。
    • Organizer
      IS-MPMI XVII congress
    • Place of Presentation
      Portland, U.S.A.
    • Year and Date
      2016-07-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナのユビキチンリガーゼPUB4はCERK1との相互作用を介して免疫 応答を制御する2016

    • Author(s)
      出崎能丈、高橋昌平、小泉春樹、三浦駿希、八嶋航平、石橋裕子、 紀藤圭治、鳴坂真理、鳴坂義弘、賀来華江、渋谷直人
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山県岡山市
    • Year and Date
      2016-03-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Evaluation of membrane traffic components involved in the MAMP-induced callose deposition in Arabidopsis2016

    • Author(s)
      K. Yashima, M. Kohari, T. Ueda, I. Hara-Nishimura, Y. Desaki, H. Kaku, N. Shibuya
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手県盛岡市
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] An E3 ubiquitin ligase, PUB4, regulates immune signaling through the interaction with Arabidopsis CERK12016

    • Author(s)
      S. Takahashi, H. Koizumi, T. Miura, K. Yashima, Y. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, Y. Desaki, H. Kaku and N. Shibuya
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手県盛岡市
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Membrane traffic components involved in MAMP-triggered callose accumulation in Arabidopsis2015

    • Author(s)
      Y. Desaki, K. Yashima, M.Kohari, T. Ueda, H. Kaku, N. Shibuya
    • Organizer
      36th New Phytologist Symposium, “Cell biology at the plant–microbe interface
    • Place of Presentation
      Germany, munich
    • Year and Date
      2015-11-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of immune signaling by an E3 ubiquitin ligase that interacts with Arabidopsis CERK12015

    • Author(s)
      Y. Desaki, S. Takahashi, K. yashima, H. Koizumi, T. Miura, H. Ishibashi, K. Kito, M. Narusaka, Y. Narusaka, H. Kaku and N. Shibuya
    • Organizer
      11th Japan-US seminar: Molecular contact points in host-pathogen co-evolution
    • Place of Presentation
      香川県高松市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] CERK1と相互作用するE3ユビキチンリガーゼPUB4による免疫シグナリン グ制御2015

    • Author(s)
      高橋昌平、三浦駿希、八嶋航平、鳴坂真理、鳴坂義弘、出崎能丈、賀来華江、 渋谷直人
    • Organizer
      平成27年度植物感染生理談話会
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2015-08-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物免疫応 答としてのカロース蓄積に関わる膜交通系因子の解析2015

    • Author(s)
      小針政輝、八嶋航平、上田貴志、出崎能丈、賀来華江、渋谷直人
    • Organizer
      平成27年度植物感染生理談話会
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2015-08-24
    • Related Report
      2015 Research-status Report
  • [Presentation] CERK1によ るリン酸化を介したPUB4の機能制御2015

    • Author(s)
      小泉春樹、石橋裕子、紀藤圭治、出崎能丈、賀来華江、渋谷直人
    • Organizer
      平成27年度植物感染生理談話会
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2015-08-24
    • Related Report
      2015 Research-status Report
  • [Presentation] CERK1と相互作用するE3ユビキチンリガーゼの機能解析2015

    • Author(s)
      高橋昌平、中島正登、八島航平、須藤健吉、小泉春樹、三浦駿希、紀藤圭治、鳴坂真理、鳴坂義弘、出崎能丈、賀来華江、渋谷直人
    • Organizer
      第56回 日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都・世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] キチン受容体キナーゼCERK1のユビキチン化部位の同定と機能解析2015

    • Author(s)
      中島正登、渋谷匡俊、田中 優太、椎野 聖大、紀藤圭治、出崎能丈、賀来華江、渋谷直人
    • Organizer
      第56回 日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都・世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] CERK1と相互作用するE3ユビキチンリガーゼの機能解析2014

    • Author(s)
      出崎能丈, 十文字純一, 竹田潤, 鈴木丸陽, 中島正登, 高橋昌平, 須藤健吉, 八嶋航平, 賀来華江, 澁谷直人
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学(神奈川県・川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] CERK1と相互作用するE3ユビキチンリガーゼの機能解析2014

    • Author(s)
      出崎能丈、十文字純一、竹田潤、鈴木丸陽、中島正登、高橋昌平、八嶋航平、須藤健吉、賀来華江、渋谷直人
    • Organizer
      平成26年度植物感染生理談話会
    • Place of Presentation
      宮城県・仙台市
    • Year and Date
      2014-08-06 – 2014-08-08
    • Related Report
      2014 Research-status Report
  • [Presentation] CHARACTERIZATION OF AN ARABIDOPSIS CERK1-INTERACTING E3 UBIQUITIN LIGASE2014

    • Author(s)
      Y. Desaki, J. Jumonji, J. Takeda, M. Suzuki, M. Nakashima, S. Takahashi, K. Yashima, K. Suto, H. Kaku, N. Shibuya
    • Organizer
      16th IS-MPMI
    • Place of Presentation
      ロードス(ギリシャ)
    • Year and Date
      2014-07-06 – 2014-07-10
    • Related Report
      2014 Research-status Report
  • [Presentation] CERK1と相互作用するE3ユビキチンリガーゼの機能解析2014

    • Author(s)
      出崎能丈、十文字純一、竹田潤、鈴木丸陽、中島正登、高橋昌平、八嶋航平、須藤健吉、賀来華江、渋谷直人
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Research-status Report
  • [Book] Research Progress in Oligosaccharins2016

    • Author(s)
      T. Shinya, Y. Desaki, N. Shibuya. et al, (Editor: Du, Y. Yin, H.)
    • Total Pages
      95
    • Publisher
      Springer-Verlag New York
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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