| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In plant, zinc (Zn) is an essential micronutrient that functions as a catalytic and structural cofactor for many proteins. Until now, various Zn transporters that are localized at the plasma membrane or vacuolar membrane have been reported in plants. However, Zn must
be properly distributed in each subcellular compartment to maintain Zn homeostasis.
This study was done to determine the biochemical and physiological function of MTP12 (metal tolerance protein 12) in Arabidopsis thaliana. I found that MTP12 is expressed throughout the plants, interacts with MTP5 in the cis-Golgi and Zn transport activity of MTP12 is MTP5 dependent. These results strongly suggested that AtMTP12 forms a heteromeric functional complex with AtMTP5 to transport Zn into the cis-Golgi. In addition, involvement of AtMTP12 in ER stress response was also assessed using AtMTP12 knockout plants.
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