Analysis of physiological function of zinc transporter MTP12 for ER stress in Arabidopsis thaliana

• Project/Area Number: 26850037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Nagoya University
• Principal Investigator: Fujiwara Takashi 名古屋大学, 生命農学研究科, 研究員 (50724499)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 亜鉛輸送体 / ゴルジ体 / シロイヌナズナ / 亜鉛

Outline of Final Research Achievements

In plant, zinc (Zn) is an essential micronutrient that functions as a catalytic and structural cofactor for many proteins. Until now, various Zn transporters that are localized at the plasma membrane or vacuolar membrane have been reported in plants. However, Zn must
be properly distributed in each subcellular compartment to maintain Zn homeostasis.
This study was done to determine the biochemical and physiological function of MTP12 (metal tolerance protein 12) in Arabidopsis thaliana. I found that MTP12 is expressed throughout the plants, interacts with MTP5 in the cis-Golgi and Zn transport activity of MTP12 is MTP5 dependent. These results strongly suggested that AtMTP12 forms a heteromeric functional complex with AtMTP5 to transport Zn into the cis-Golgi. In addition, involvement of AtMTP12 in ER stress response was also assessed using AtMTP12 knockout plants.

Research Products

• [Journal Article] The PP-motif in luminal loop 2 of ZnT transporters plays a pivotal role in TNAP activation (2016)
  • Author(s): Fujimoto S, Tsuji T, Fujiwara T, Takeda T, Merriman C, Fukunaka A, Nishito Y, Fu D, Hoch E, Sekler I, Fukue K, Miyamae Y, Masuda S, Nagao M, Kambe T
  • Journal Title: Biochem. J Volume: 473 Issue: 17 Pages: 2611-2621
  • DOI: 10.1042/bcj20160324
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A high molecular mass zinc transporter MTP12 forms a functional heteromeric complex with MTP5 in the Golgi in Arabidopsis thaliana. (2015)
  • Author(s): Fujiwara T, Kawachi M, Sato Y, Mori H, Kutsuna N, Hasezawa S, Maeshima M
  • Journal Title: FEBS Journal Volume: 印刷中 Issue: 10 Pages: 1965-1979
  • DOI: 10.1111/febs.13252
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] シロイヌナズナ亜鉛輸送体AtMTP12の機能解析 (2015)
  • Author(s): 藤原崇志、河内美樹、前島正義
  • Organizer: メタロバイオサイエンス研究会2015
  • Place of Presentation: 名古屋国際センター
  • Year and Date: 2015-08-27
• [Presentation] シロイヌナズナ亜鉛輸送体ZIP13 の花粉管伸長における役割 (2015)
  • Author(s): 藤田早紀，長崎（武内）菜穂子，藤原崇志，深尾陽一朗，前島正義，河内美樹
  • Organizer: 第56回日本植物生理学会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18