| Project/Area Number |
26850041
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | muconic acid / protocatechuic acid / decarboxylase / bio-based monomer / lignin / Protocatechuic acid / Decarboxylase / Flavin prenyltransferase / cis,cis-muconic acid / Lignin / Pseudomonas putida / プロトカテク酸脱炭酸酵素 / ムコン酸 |
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| Outline of Final Research Achievements |
In this work, the mechanism of protocatechuate decarboxylation was investigated to develop an efficient production of a platform chemical, cis,cis-muconic acid (ccMA). The expression of KpdB, annotated as a 4-hydroxybenzoate decarboxylase subunit B, with protocatechuate decarboxylase (Pdc) could enhance Pdc activity, and it was revealed that KpdB is a flavin prenyltransferase and produces prenylated flavin mononucleotide as a cofactor of Pdc. By using the enhanced Pdc activity, a new ccMA producing bacterial strain that can produce ccMA from not only lignin model compounds but also rice straw lignin-derived aromatics was developed.
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