| Project/Area Number |
26850042
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | タンパク質工学 / 結晶構造解析 / バイオセンサー |
|---|
| Outline of Final Research Achievements |
Root nodule bacteria control expression levels of protein involved in nitrate assimilation, and the sensing is regulated by protein complex of NasS and NasT. This project tried to reveal nitrate sensing mechanism of NasS-NasT at molecular level, and develop bio-sensor of nitrate in the cells by utilizing NasS-NasT.
To clarify structure and function relationships of NasS-NasT, crystallographic approach was attempted. After successful construction of expression and purification system of NasS-NasT, these proteins were subjected to crystallization and X-ray analysis. Finally, novel crystal structure of NasS was solved.
In this project, sNOOOpy, sensor for NO3/NO2 in physiology, was developed. This system utilizing NasS-NasT protein enabled us to visualize the changes of nitrate and nitrite level in mammalian cells.
|
