| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Secondary metabolites (SMs) produced by actinomycetes are great resources for therapeutic application. Actinomycetes are suggested to possess more than 20 of cryptic gene clusters for SMs production. Our study focus on co-culture method between actinomycetes and mycolic acid-containing bacteria (MACB), that had been shown to activate the cryptic gene clusters of actinomycetes. We used biosynthetic gene cluster of a ribosomal synthesized peptide antibiotic as a model to elucidate the mechanism of activation by stimulation of MACB.
Within this specific research project, godA promoter (PgodA) was identified using genetic analyses and colorimetric reporter assay. The sequence of PgodA showed consensus of HrdB. Transcriptomic analyses of co-culture between Streptomyces coelicolor and MACB showed global variations of gene expression by S. coelicolor, suggesting a possibility that overproduction of SMs are as the result of flux balance variations of whole cell metabolism.
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