| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We attempted to identify a long-chain fatty acid transporter using fatty acid-producing strains of Corynebacterium glutamicum. After selecting four candidate genes for the transporter, we conducted single and multiple disruptions of these genes in the fatty acid-producing strain. The resulting quadruple-deletion strain exhibited severely impaired growth under culture conditions that enable extracellular production of fatty acids. However, such impairment was not observed under the conditions that do not permit fatty acid production. Similar results were observed only for the single-deletion strain in which a certain gene was disrupted. From these results, this gene is regarded to be the prospect involved in fatty acid transport. At present, there remains the possibility that one or more of the other candidates are also involved in the transport.
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