| Project/Area Number |
26850052
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
Inoue Kengo 宮崎大学, 農学部, 准教授 (70581304)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 鉄還元菌 / Geobacter / 細胞外電子伝達 / Ⅱ型分泌システム / シトクロムc / ヘム修飾 / c型シトクロム |
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| Outline of Final Research Achievements |
Geobacter sulfurreducens is a key player in current production by mixed-culture microbial fuel cells. Among known essential genes for optimal current production, omcZ is the only c-type cytochrome. To reveal the molecular mechanisms of OmcZ secretion and maturation process, we have investigated type-II secretion system (T2SS) by which OmcZ is proposed to be secreted. In G. sulfurreducens genome, there are two T2SS gene clusters (T2SS-I and -II, respectively). We generated disruption mutants of these T2SS-related genes and investigated their cytochrome production and capability of current production in microbial fuel cell. According to the cytochrome production profile results, T2SS-I disrupted mutant could not produce maturated OmcZ but T2SS-II disrupted mutant did not show difference from wild-type G. sulfurreducens strain. However, both mutants could produce current in microbial fuel cell as well as wild-type.
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