Fundamental research for advanced use of polyethylene terephthalate-degrading enzymes
Project/Area Number |
26850053
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | Kyoto University |
Principal Investigator |
Yoshida Shosuke 京都大学, 白眉センター, 特定准教授 (80610766)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | ポリエチレンテレフタレート / PETase / MHETase / 加水分解酵素 / ゲノム解析 / モノヒドロキシエチルテレフタレート / ゲノム / トランスクリプトーム / エステラーゼ / ブレビバチルス |
Outline of Final Research Achievements |
For the advanced use of two novel enzymes PET hydrolase (PETase) and mono (hydroxyethyl) terephthalate (MHET) hydrolase (MHETase) found from a poly(ethylene terephthalate) (PET)-degrading bacterium, these enzymes have been functionally identified, developed for the abundant soluble protein expression, and functionally improved. As a result, detailed functions of PETase has been identified and PETase mutants with improved thermal stability has been obtained.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Response to Comment on “A bacterium that degrades and assimilates poly(ethylene terephthalate)2016
Author(s)
Yoshida, S. Hiraga, K. Takehana, T. Taniguchi, I. Yamaji, H. Maeda, Y. Toyohara, K. Miyamoto, K. Kimura, Y., Oda, K.
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Journal Title
Science
Volume: 353
Issue: 6301
Pages: 759-759
DOI
Related Report
Peer Reviewed
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