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Elucidation and control of interaction between bacterial cells and electrodes towards improvement of bioelectrochemical systems

Research Project

Project/Area Number 26850056
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

Kouzuma Atsushi  東京薬科大学, 生命科学部, 助教 (20634471)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords微生物電気化学 / 細胞外電子伝達 / 微生物燃料電池 / 遺伝子発現 / バイオフィルム / 発現制御
Outline of Final Research Achievements

In this study, we investigated molecular mechanisms underlying interactions between Shewanella oneidensis MR-1 and electrodes in bioelectrochemical systems. The observation of biofilm formation processes using a confocal laser scanning microscope and electrochemical flow cells revealed that MR-1 forms structurally different biofilms on graphite electrodes depending on the electrode potential. We also found that the electrode potential affects the gene expression and metabolic profiles of MR-1, and elucidated the molecular mechanism underlying the electrode potential sensing of this strain.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (10 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] CRP regulates D-lactate oxidation in Shewanella oneidensis MR-12017

    • Author(s)
      Takuya Kasai, Atsushi Kouzuma, Kazuya Watanabe
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 869-869

    • DOI

      10.3389/fmicb.2017.00869

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Catabolic and regulatory systems in Shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells2015

    • Author(s)
      Kouzuma A, Kasai T, Hirose A, Watanabe K
    • Journal Title

      Front Microbiol

      Volume: 6 Pages: 609-609

    • DOI

      10.3389/fmicb.2015.00609

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metabolic Characteristics of a Glucose-Utilizing Shewanella oneidensis Strain Grown under Electrode-Respiring Conditions2015

    • Author(s)
      Nakagawa G, Kouzuma A, Hirose A, Kasai T, Yoshida G, Watanabe K
    • Journal Title

      PLoS One

      Volume: 10 Issue: 9 Pages: e0138813-e0138813

    • DOI

      10.1371/journal.pone.0138813

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcriptional mechanisms for differential expression of outer membrane cytochrome genes omcA and mtrC in Shewanella oneidensis MR-1.2015

    • Author(s)
      Kasai T, Kouzuma A, Nojiri H, Watanabe K.
    • Journal Title

      BMC Microbiology

      Volume: 15 Issue: 1 Pages: 68-68

    • DOI

      10.1186/s12866-015-0406-8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Shewanella oneidensis MR-1株におけるCRP依存的D-乳酸代謝制御機構2017

    • Author(s)
      笠井拓哉、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] Shewanella oneidensis MR-1株のMetRによる嫌気呼吸制御系の解明2017

    • Author(s)
      茂木久恵、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] Methionine stimulates anaerobic respiration in Shewanella oneidensis MR-12016

    • Author(s)
      Hisae Mogi, Atsushi Kouzuma, and Kazuya Watanabe
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      モントリオール
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Electrode potential-dependent energy conservation in Shewanella oneidensis MR-12016

    • Author(s)
      Atsumi Hirose, Atsushi Kouzuma, Motohide Aoki, Tomonari Umemura, and Kazuya Watabane
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      モントリオール
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Shewanella oneidensis MR -1 株のエネルギー獲得系の電極電位応答機構2016

    • Author(s)
      廣瀬篤弥、高妻篤史、青木元秀、梅村知也、渡邉一哉
    • Organizer
      環境バイオテクノロジー学会2016年度大会
    • Place of Presentation
      サテライトキャンパスひろしま
    • Year and Date
      2016-06-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] Acidithiobacillus ferrooxidans の窒素固定制御系の解析2016

    • Author(s)
      山田祥平、高妻篤史、渡邉一哉
    • Organizer
      環境バイオテクノロジー学会2016年度大会
    • Place of Presentation
      サテライトキャンパスひろしま
    • Year and Date
      2016-06-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] Metabolic and transcriptional characteristics of an engineered glucose-utilizing Shewanella oneidensis strain grown under electrode-respiring conditions2015

    • Author(s)
      Atsushi Kouzuma, Gen Nakagawa, Kazuya Watanabe
    • Organizer
      The 5th International Meeting on Microbial Electrochemistry and Technologies
    • Place of Presentation
      アリゾナ州立大学
    • Year and Date
      2015-10-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Transcriptional regulation of outer membrane cytochrome genes omcA and mtrC by two CRP- dependent promoters in Shewanella oneidensis MR-12015

    • Author(s)
      Takuya Kasai, Atsushi Kouzuma, Kazuya Watanabe
    • Organizer
      The 5th International Meeting on Microbial Electrochemistry and Technologies
    • Place of Presentation
      アリゾナ州立大学
    • Year and Date
      2015-10-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Isolation and electrochemical cultivation of a novel iron-oxidizing bacterium NU-1 affiliated with the genus Acidithiobacillus2015

    • Author(s)
      Nagayoshi Ueoka, Atsushi Kouzuma, Kazuya Watanabe
    • Organizer
      The 5th International Meeting on Microbial Electrochemistry and Technologies
    • Place of Presentation
      アリゾナ州立大学
    • Year and Date
      2015-10-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Shewanella oneidensisの電位応答のトランスクリプトーム解析2015

    • Author(s)
      廣瀬篤弥,高妻篤史,渡邉一哉
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Book] 微生物燃料電池の開発と展望(表面・界面技術ハンドブック)2016

    • Author(s)
      宮原盛雄、高妻篤史、渡邉一哉
    • Total Pages
      750
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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