Expression mechanism of cellulase and hemicellulase genes in the fungus Talaromyces cellulolyticus (formerly Acremonium cellulolyticus)
| Project/Area Number |
26850058
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | National Institute of Advanced Industrial Science and Technology |
|---|
Principal Investigator |
FUJII Tatsuya 国立研究開発法人産業技術総合研究所, 機能化学研究部門, 主任研究員 (10613549)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 糖化酵素 / セルラーゼ / タラロマイセス・セルロリティカス / アクレモニウム・セルロリティカス / 転写因子 |
|---|
| Outline of Final Research Achievements |
The expression mechanism of cellulase and xylanase genes in the filamentous fungus Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) was investigated by (i) analyzing the transcriptional factors genes which showed homology with those in other filamentous fungi, (ii) screening of the novel transcriptional factors.
We found that tacA, tctA, and Hap complex genes contribute to expression of cellulase and xylanase genes. Although the novel transcriptional factors have not been obtained, the strain which has high homologous recombination efficiency and the important promoter regions for cellulase gene expression were isolated.
|
Report
(4 results)
Research Products
(5 results)
