Project/Area Number |
26850060
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
|
Research Institution | Osaka City University (2015-2016) University of Tsukuba (2014) |
Principal Investigator |
Oinuma Ken-Ichi 大阪市立大学, 大学院医学研究科, 助教 (20635619)
|
Research Collaborator |
TAKAYA Naoki
KANEKO Tatsuo
KIZAWA Yoshie
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 芳香族ポリマー / 加水分解酵素 / Aspergillus niger / 芳香族ポリマ- |
Outline of Final Research Achievements |
Aromatic polymers are widely used in plastic industries. One property common to these polymers is their resistance to bio-degradation and, despite its importance, there are only a few reports on aromatic polymer-degrading microorganism or enzymes. In this study, we used 4CDP, a recently developed aromatic polymer, as a model substrate and screened for microorganisms or enzymes that can degrade aromatic polymers. We found that a fungus in the genus Aspergillus produces some enzyme that hydrolyzes 4CDP. Moreover, we succeeded in purifying the enzyme from an Aspergillus-originated material and identifying the gene encoding the enzyme. We expect that knowledge on this enzyme will contribute to development of aromatic polymer-recycling system, which is to be materialized in the future.
|