Screening and analysis of aromatic polymer-hydrolyzing enzymes

• Project/Area Number: 26850060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Osaka City University (2015-2016); University of Tsukuba (2014)
• Principal Investigator: Oinuma Ken-Ichi 大阪市立大学, 大学院医学研究科, 助教 (20635619)
• Research Collaborator: TAKAYA Naoki ; KANEKO Tatsuo ; KIZAWA Yoshie
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 芳香族ポリマー / 加水分解酵素 / Aspergillus niger / 芳香族ポリマ－

Outline of Final Research Achievements

Aromatic polymers are widely used in plastic industries. One property common to these polymers is their resistance to bio-degradation and, despite its importance, there are only a few reports on aromatic polymer-degrading microorganism or enzymes. In this study, we used 4CDP, a recently developed aromatic polymer, as a model substrate and screened for microorganisms or enzymes that can degrade aromatic polymers. We found that a fungus in the genus Aspergillus produces some enzyme that hydrolyzes 4CDP. Moreover, we succeeded in purifying the enzyme from an Aspergillus-originated material and identifying the gene encoding the enzyme. We expect that knowledge on this enzyme will contribute to development of aromatic polymer-recycling system, which is to be materialized in the future.

Research Products

• [Journal Article] Structural basis of the substrate recognition of hydrazidase isolated from Microbacterium sp. strain HM58-2, which catalyzes acylhydrazide compounds as its sole carbon source (2017)
  • Author(s): Akiyama T., Ishii M., Takuwa A., Oinuma K., Sasaki Y., Takaya N. and Yajima S.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 482 Issue: 4 Pages: 1007-1012
  • DOI: 10.1016/j.bbrc.2016.11.148
  • Peer Reviewed
• [Journal Article] Identification of a novel Rhizopus-specific antigen by screening with a signal sequence trap and evaluation as a possible diagnostic marker of mucormycosis (2017)
  • Author(s): Sato K., Oinuma K., Niki M., Yamagoe S., Miyazaki Y., Asai K., Yamada K., Hirata K., Kaneko Y. and Kakeya H.
  • Journal Title: Medical Mycology Volume: 印刷中 Issue: 7 Pages: 713-719
  • DOI: 10.1093/mmy/myw146
  • NAID: 120006533842
  • Peer Reviewed
• [Journal Article] Clinical characteristics of bacteremia caused by extended-spectrum beta-lactamase-producing Escherichia coli at a tertiary hospital (2017)
  • Author(s): Namikawa H., Yamada K., Fujimoto H., Oinuma K., Tochino Y., Takemoto Y., Kaneko Y., Shuto T. and Kakeya H.
  • Journal Title: Internal Medicine Volume: 印刷中
  • NAID: 130005773825
  • Peer Reviewed / Open Access
• [Journal Article] Alternative fermentation pathway of cinnamic acid production via phenyllactic acid (2016)
  • Author(s): Masuo S., Kobayashi Y., Oinuma K. and Takaya N.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 100 Issue: 20 Pages: 8701-8709
  • DOI: 10.1007/s00253-016-7623-4
  • Peer Reviewed
• [Journal Article] Draft genome sequence of Microbacterium sp. strain HM58-2, which hydrolyzes acylhydrazides (2016)
  • Author(s): Akiyama T., Ishige T., Kanesaki Y., Ito S., Oinuma K., Takaya N., Sasaki Y. and Yajima S.
  • Journal Title: Genome Announcements Volume: 4 Issue: 3
  • DOI: 10.1128/genomea.00554-16
  • Peer Reviewed / Open Access
• [Journal Article] An Intrinsic Strain of Colistin-resistant <i>Acinetobacter</i> Isolated from a Japanese Patient (2016)
  • Author(s): Takizawa E., Yamada K., Oinuma K., Sato K., Niki M., Namikawa H., Fujimoto H., Asai K., Kaneko Y. and Kakeya H.
  • Journal Title: Internal Medicine Volume: 55 Issue: 16 Pages: 2301-2306
  • DOI: 10.2169/internalmedicine.55.6807
  • NAID: 130005169382
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Genome sequence of an Acinetobacter baumannii strain carrying three acquired carbapenemase genes (2016)
  • Author(s): Oinuma K., Suzuki M., Sato K., Nakaie K., Niki M., Takizawa E., Niki M., Shibayama K., Yamada K., Kakeya H. and Kaneko Y.
  • Journal Title: Genome Announcements Volume: 4 Issue: 6
  • DOI: 10.1128/genomea.01290-16
  • Peer Reviewed / Open Access
• [Journal Article] Two unusual cases of successful treatment of hypermucoviscous Klebsiella pneumoniae invasive syndrome (2016)
  • Author(s): Namikawa H., Yamada K., Fujimoto H., Oinuma K., Tochino Y., Takemoto Y., Kaneko Y., Shuto T. and Kakeya H.
  • Journal Title: BMC Infectious Diseases Volume: 16 Issue: 1 Pages: 680-680
  • DOI: 10.1186/s12879-016-2011-3
  • Peer Reviewed / Open Access
• [Presentation] ゲノム情報に基づくアシネトバクター属臨床分離株の分子疫学解析 (2017)
  • Author(s): 老沼 研一, 鈴木 仁人, 佐藤 佳奈子, 中家 清隆, 滝沢 恵津子, 仁木 誠, 仁木 満美子, 山田 康一, 柴山 恵吾, 掛屋 弘, 金子 幸弘
  • Organizer: 第91回日本感染症学会総会・学術講演会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2017-04-06
• [Presentation] POT法およびMLST法によるアシネトバクター属臨床分離株の遺伝子型解析 (2017)
  • Author(s): 老沼 研一, 鈴木 仁人, 佐藤 佳奈子, 中家 清隆, 滝沢 恵津子, 仁木 誠, 仁木 満美子, 山田 康一, 柴山 恵吾, 掛屋 弘, 金子 幸弘
  • Organizer: 第51回緑膿菌感染症研究会学術集会
  • Place of Presentation: レンブラントホテル大分（大分県大分市）
  • Year and Date: 2017-02-10
• [Presentation] 当院で血液培養より分離されたアシネトバクター属細菌の分子疫学解析 (2017)
  • Author(s): 金子 幸弘, 老沼 研一, 佐藤 佳奈子, 鈴木 仁人, 柴山 恵吾, 滝沢 恵津子, 仁木 誠, 中家 清隆, 仁木 満美子, 山田 康一, 掛屋 弘
  • Organizer: 第28回日本臨床微生物学会総会・学術集会
  • Place of Presentation: 長崎ブリックホール, 長崎新聞文化ホール, 長崎県医師会館（長崎県長崎市）
  • Year and Date: 2017-01-20
• [Presentation] アシネトバクター属臨床分離株の薬剤感受性試験と分子疫学解析 (2016)
  • Author(s): 老沼 研一, 佐藤 佳奈子, 鈴木 仁人, 柴山 恵吾, 滝沢 恵津子, 仁木 誠, 中家 清隆, 仁木 満美子, 山田 康一, 掛屋 弘, 金子 幸弘
  • Organizer: 第59回日本感染症学会中日本地方会学術集会
  • Place of Presentation: 沖縄コンベンションセンター, カルチャーリゾートフェストーネ（沖縄県宜野湾市）
  • Year and Date: 2016-11-24
• [Presentation] マウスモデルを用いた侵襲性肺ムーコル症に対する新規診断系の検討 (2016)
  • Author(s): 金子 幸弘, 佐藤 佳奈子, 老沼 研一, 仁木 満美子, 山田 康一, 山越 智, 宮崎 義継, 掛屋 弘
  • Organizer: 第59回日本感染症学会中日本地方会学術集会
  • Place of Presentation: 沖縄コンベンションセンター, カルチャーリゾートフェストーネ（沖縄県宜野湾市）
  • Year and Date: 2016-11-24
• [Presentation] 侵襲性肺ムーコル症マウスモデルにおけるRhizopus oryzae特異的分泌抗原の有用性の検討 (2016)
  • Author(s): 佐藤 佳奈子, 老沼 研一, 山田 康一, 掛屋 弘, 金子 幸弘
  • Organizer: 第60回日本医真菌学会総会・学術集会
  • Place of Presentation: 東京都立産業貿易センター台東館（東京都台東区）
  • Year and Date: 2016-10-01
• [Presentation] Aspergillus calidoustus重複感染が確認されたLichtheimia ramosaによる消化管型ムーコル症の一例 (2016)
  • Author(s): 水谷 哲, 老沼 研一, 寺地 つね子, 掛屋 弘, 金子 幸弘
  • Organizer: 第60回日本医真菌学会総会・学術集会
  • Place of Presentation: 東京都立産業貿易センター台東館（東京都台東区）
  • Year and Date: 2016-10-01
• [Presentation] 遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析 (2016)
  • Author(s): 宮川 寛規, 川口 秀夫, 荻野 千秋, 老沼 研一, 高谷 直樹, 近藤 昭彦
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山国際会議場, ANAクラウンプラザホテル富山（富山県富山市）
  • Year and Date: 2016-09-28
• [Presentation] Discovery and analysis of a novel Rhizopus antigen as a potential diagnostic marker for mucormycosis (2016)
  • Author(s): Oinuma K., Sato K., Niki M., Kakeya H. and Kaneko Y.
  • Organizer: 2016 International Symposium on New Frontiers in Microbiology and Biotechnology
  • Place of Presentation: 筑波大学（茨城県つくば市）
  • Year and Date: 2016-07-06
  • Int'l Joint Research / Invited
• [Presentation] Aspergillus niger由来芳香族ポリエステル分解酵素の発見と解析 (2015)
  • Author(s): 老沼 研一、金子 達雄、高谷 直樹
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス（岡山県岡山市）
  • Year and Date: 2015-03-28