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Screening and analysis of aromatic polymer-hydrolyzing enzymes

Research Project

Project/Area Number 26850060
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionOsaka City University (2015-2016)
University of Tsukuba (2014)

Principal Investigator

Oinuma Ken-Ichi  大阪市立大学, 大学院医学研究科, 助教 (20635619)

Research Collaborator TAKAYA Naoki  
KANEKO Tatsuo  
KIZAWA Yoshie  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords芳香族ポリマー / 加水分解酵素 / Aspergillus niger / 芳香族ポリマ-
Outline of Final Research Achievements

Aromatic polymers are widely used in plastic industries. One property common to these polymers is their resistance to bio-degradation and, despite its importance, there are only a few reports on aromatic polymer-degrading microorganism or enzymes. In this study, we used 4CDP, a recently developed aromatic polymer, as a model substrate and screened for microorganisms or enzymes that can degrade aromatic polymers. We found that a fungus in the genus Aspergillus produces some enzyme that hydrolyzes 4CDP. Moreover, we succeeded in purifying the enzyme from an Aspergillus-originated material and identifying the gene encoding the enzyme. We expect that knowledge on this enzyme will contribute to development of aromatic polymer-recycling system, which is to be materialized in the future.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (18 results)

All 2017 2016 2015

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Structural basis of the substrate recognition of hydrazidase isolated from Microbacterium sp. strain HM58-2, which catalyzes acylhydrazide compounds as its sole carbon source2017

    • Author(s)
      Akiyama T., Ishii M., Takuwa A., Oinuma K., Sasaki Y., Takaya N. and Yajima S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 482 Issue: 4 Pages: 1007-1012

    • DOI

      10.1016/j.bbrc.2016.11.148

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a Novel Rhizopus-specific Antigen by Screening with a Signal Sequence Trap and Evaluation as a Possible Diagnostic Marker of Mucormycosis2017

    • Author(s)
      Sato K., Oinuma K., Niki M., Yamagoe S., Miyazaki Y., Asai K., Yamada K., Hirata K., Kaneko Y. and Kakeya H.
    • Journal Title

      Medical Mycology

      Volume: 55 Issue: 7 Pages: 713-719

    • DOI

      10.1093/mmy/myw146

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2020099

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical characteristics of bacteremia caused by extended-spectrum beta-lactamase-producing Escherichia coli at a tertiary hospital2017

    • Author(s)
      Namikawa H., Yamada K., Fujimoto H., Oinuma K., Tochino Y., Takemoto Y., Kaneko Y., Shuto T. and Kakeya H.
    • Journal Title

      Internal Medicine

      Volume: 印刷中

    • NAID

      130005773825

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alternative fermentation pathway of cinnamic acid production via phenyllactic acid2016

    • Author(s)
      Masuo S., Kobayashi Y., Oinuma K. and Takaya N.
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 100 Issue: 20 Pages: 8701-8709

    • DOI

      10.1007/s00253-016-7623-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Draft genome sequence of Microbacterium sp. strain HM58-2, which hydrolyzes acylhydrazides2016

    • Author(s)
      Akiyama T., Ishige T., Kanesaki Y., Ito S., Oinuma K., Takaya N., Sasaki Y. and Yajima S.
    • Journal Title

      Genome Announcements

      Volume: 4 Issue: 3

    • DOI

      10.1128/genomea.00554-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An Intrinsic Strain of Colistin-resistant <i>Acinetobacter</i> Isolated from a Japanese Patient2016

    • Author(s)
      Takizawa E., Yamada K., Oinuma K., Sato K., Niki M., Namikawa H., Fujimoto H., Asai K., Kaneko Y. and Kakeya H.
    • Journal Title

      Internal Medicine

      Volume: 55 Issue: 16 Pages: 2301-2306

    • DOI

      10.2169/internalmedicine.55.6807

    • NAID

      130005169382

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome sequence of an Acinetobacter baumannii strain carrying three acquired carbapenemase genes2016

    • Author(s)
      Oinuma K., Suzuki M., Sato K., Nakaie K., Niki M., Takizawa E., Niki M., Shibayama K., Yamada K., Kakeya H. and Kaneko Y.
    • Journal Title

      Genome Announcements

      Volume: 4 Issue: 6

    • DOI

      10.1128/genomea.01290-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two unusual cases of successful treatment of hypermucoviscous Klebsiella pneumoniae invasive syndrome2016

    • Author(s)
      Namikawa H., Yamada K., Fujimoto H., Oinuma K., Tochino Y., Takemoto Y., Kaneko Y., Shuto T. and Kakeya H.
    • Journal Title

      BMC Infectious Diseases

      Volume: 16 Issue: 1 Pages: 680-680

    • DOI

      10.1186/s12879-016-2011-3

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ゲノム情報に基づくアシネトバクター属臨床分離株の分子疫学解析2017

    • Author(s)
      老沼 研一, 鈴木 仁人, 佐藤 佳奈子, 中家 清隆, 滝沢 恵津子, 仁木 誠, 仁木 満美子, 山田 康一, 柴山 恵吾, 掛屋 弘, 金子 幸弘
    • Organizer
      第91回日本感染症学会総会・学術講演会
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2017-04-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] POT法およびMLST法によるアシネトバクター属臨床分離株の遺伝子型解析2017

    • Author(s)
      老沼 研一, 鈴木 仁人, 佐藤 佳奈子, 中家 清隆, 滝沢 恵津子, 仁木 誠, 仁木 満美子, 山田 康一, 柴山 恵吾, 掛屋 弘, 金子 幸弘
    • Organizer
      第51回緑膿菌感染症研究会学術集会
    • Place of Presentation
      レンブラントホテル大分(大分県大分市)
    • Year and Date
      2017-02-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 当院で血液培養より分離されたアシネトバクター属細菌の分子疫学解析2017

    • Author(s)
      金子 幸弘, 老沼 研一, 佐藤 佳奈子, 鈴木 仁人, 柴山 恵吾, 滝沢 恵津子, 仁木 誠, 中家 清隆, 仁木 満美子, 山田 康一, 掛屋 弘
    • Organizer
      第28回日本臨床微生物学会総会・学術集会
    • Place of Presentation
      長崎ブリックホール, 長崎新聞文化ホール, 長崎県医師会館(長崎県長崎市)
    • Year and Date
      2017-01-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] アシネトバクター属臨床分離株の薬剤感受性試験と分子疫学解析2016

    • Author(s)
      老沼 研一, 佐藤 佳奈子, 鈴木 仁人, 柴山 恵吾, 滝沢 恵津子, 仁木 誠, 中家 清隆, 仁木 満美子, 山田 康一, 掛屋 弘, 金子 幸弘
    • Organizer
      第59回日本感染症学会中日本地方会学術集会
    • Place of Presentation
      沖縄コンベンションセンター, カルチャーリゾートフェストーネ(沖縄県宜野湾市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウスモデルを用いた侵襲性肺ムーコル症に対する新規診断系の検討2016

    • Author(s)
      金子 幸弘, 佐藤 佳奈子, 老沼 研一, 仁木 満美子, 山田 康一, 山越 智, 宮崎 義継, 掛屋 弘
    • Organizer
      第59回日本感染症学会中日本地方会学術集会
    • Place of Presentation
      沖縄コンベンションセンター, カルチャーリゾートフェストーネ(沖縄県宜野湾市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 侵襲性肺ムーコル症マウスモデルにおけるRhizopus oryzae特異的分泌抗原の有用性の検討2016

    • Author(s)
      佐藤 佳奈子, 老沼 研一, 山田 康一, 掛屋 弘, 金子 幸弘
    • Organizer
      第60回日本医真菌学会総会・学術集会
    • Place of Presentation
      東京都立産業貿易センター台東館(東京都台東区)
    • Year and Date
      2016-10-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] Aspergillus calidoustus重複感染が確認されたLichtheimia ramosaによる消化管型ムーコル症の一例2016

    • Author(s)
      水谷 哲, 老沼 研一, 寺地 つね子, 掛屋 弘, 金子 幸弘
    • Organizer
      第60回日本医真菌学会総会・学術集会
    • Place of Presentation
      東京都立産業貿易センター台東館(東京都台東区)
    • Year and Date
      2016-10-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析2016

    • Author(s)
      宮川 寛規, 川口 秀夫, 荻野 千秋, 老沼 研一, 高谷 直樹, 近藤 昭彦
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場, ANAクラウンプラザホテル富山(富山県富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Discovery and analysis of a novel Rhizopus antigen as a potential diagnostic marker for mucormycosis2016

    • Author(s)
      Oinuma K., Sato K., Niki M., Kakeya H. and Kaneko Y.
    • Organizer
      2016 International Symposium on New Frontiers in Microbiology and Biotechnology
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Year and Date
      2016-07-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Aspergillus niger由来芳香族ポリエステル分解酵素の発見と解析2015

    • Author(s)
      老沼 研一、金子 達雄、高谷 直樹
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県岡山市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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