Structure and function of chitinase-like proteins involved in cell wall biosynthesis.

• Project/Area Number: 26850066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kinki University
• Principal Investigator: OHNUMA Takayuki 近畿大学, 農学部, 准教授 (60446482)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: chitinase-like protein / キチナーゼ様タンパク質

Outline of Final Research Achievements

AtCTL1, AtCTL2 and OsCTL1 are CTL, chitinase-like, proteins from Arabidopsis and rice (Oryza sativa). They consist of an N-terminal signal sequence, transmembrane region, GH19 chitinase-like domain, and C-teminal extension. We expressed the GH19 chitinase-like domains of AtCTl1 and AtCTL2 as fusion protein with glutathione S-transferase and SUMO protein, respectively, and purified them to homogeneity as judged by SDS/PAGE.