Analysis and applications of metagenomic high-performance beta-glucosidases

• Project/Area Number: 26850067
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Matsuzawa Tomohiko 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (10711971)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: メタゲノム / グルコシダーゼ / バイオマス / 酵素 / 糖質加水分解酵素 / 生成物阻害

Outline of Final Research Achievements

Novel β-glucosidases, which showed tolerance to product-inhibition and high temperature, were isolated from environmental metagenomes. A novel β-glucosidase showed unique enzymatic properties and had positive effects on the enzymatic saccharification of lignocellulosic biomass. In addition, thermostability of the β-glucosidase was improved by site-directed mutagenesis.

Research Products

• [Journal Article] Improved thermostability of a metagenomic glucose-tolerant β-glycosidase based on its X-ray crystal structure (2017)
  • Author(s): Matsuzawa Tomohiko、Watanabe Masahiro、Yaoi Katsuro
  • Journal Title: Applied Microbiology and Biotechnology Volume: 101 Issue: 23-24 Pages: 8353-8363
  • DOI: 10.1007/s00253-017-8525-9
  • Peer Reviewed
• [Journal Article] Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library. (2017)
  • Author(s): Tomohiko Matsuzawa, Katsuro Yaoi
  • Journal Title: Applied Microbiology and Biotechnology Volume: 101 Issue: 2 Pages: 633-646
  • DOI: 10.1007/s00253-016-7803-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Screening, identification, and characterization of α-xylosidase from a soil metagenome (2016)
  • Author(s): Matsuzawa T, Kimura N, Suenaga H, Yaoi K.
  • Journal Title: J BIOSCI BIOENG Volume: 122 Issue: 4 Pages: 393-399
  • DOI: 10.1016/j.jbiosc.2016.03.012
  • NAID: 40020966071
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity and transglycosylation activity of metagenomic β-glucosidase Td2F2. (2016)
  • Author(s): Tomohiko Matsuzawa, Toshinori Jo, Taku Uchiyama, Jenny A Manninen, Takatoshi Arakawa, Kentaro Miyazaki, Shinya Fushinobu, Katsuro Yaoi
  • Journal Title: FEBS Journal Volume: - Issue: 12 Pages: 2340-2353
  • DOI: 10.1111/febs.13743
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ユニークな新規糖質加水分解酵素の単離と解析 (2017)
  • Author(s): 松沢智彦
  • Organizer: 平成29年度 日本応用糖質科学会 東日本支部 若手奨励賞 受賞講演
  • Invited
• [Presentation] メタゲノムから単離した生成物阻害耐性を有するβ-グルコシダーゼの解析と高機能化 (2017)
  • Author(s): 松沢智彦、渡邊真宏、矢追克郎
  • Organizer: 日本応用糖質科学会 平成29年度大会
• [Presentation] メタゲノム由来 新規有用酵素の探索・同定・精密解析・高機能化 (2017)
  • Author(s): 松沢智彦
  • Organizer: 2017年度生物工学若手研究者の集い（若手会）夏のセミナー
• [Presentation] メタゲノム由来β-グルコシダーゼTd2F2の生成物阻害耐性メカニズムの解析 (2015)
  • Author(s): 松沢智彦, 内山 拓, 城 俊徳, Jenny Manninen, 伏信進矢, 矢追克郎
  • Organizer: 日本農芸化学会 2015年度大会
  • Place of Presentation: 岡山大学（岡山市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 生成物阻害耐性β-グルコシダーゼTd2F2の生成物阻害耐性機構の解析 (2014)
  • Author(s): 松沢智彦, 内山 拓, 城 俊徳, 伏信進矢, 矢追克郎
  • Organizer: 日本応用糖質科学会 平成26年度大会
  • Place of Presentation: 朱鷺メッセ（新潟市）
  • Year and Date: 2014-09-24 – 2014-09-26
• [Patent(Industrial Property Rights)] 生成物阻害耐性および基質阻害耐性を有するβ－グルコシダーゼ、それをコードする遺伝子 (2017)
  • Inventor(s): 松沢智彦、渡邊真宏、矢追克郎
  • Industrial Property Rights Holder: 松沢智彦、渡邊真宏、矢追克郎
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-006497
  • Filing Date: 2017-01-18