Analysis and applications of metagenomic high-performance beta-glucosidases
| Project/Area Number |
26850067
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Matsuzawa Tomohiko 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (10711971)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | メタゲノム / グルコシダーゼ / バイオマス / 酵素 / 糖質加水分解酵素 / 生成物阻害 |
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| Outline of Final Research Achievements |
Novel β-glucosidases, which showed tolerance to product-inhibition and high temperature, were isolated from environmental metagenomes. A novel β-glucosidase showed unique enzymatic properties and had positive effects on the enzymatic saccharification of lignocellulosic biomass. In addition, thermostability of the β-glucosidase was improved by site-directed mutagenesis.
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Report
(5 results)
Research Products
(10 results)
