Proposals for definition of strigolactones and description of stereochemistry in biological activities

• Project/Area Number: 26850069
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bioorganic chemistry
• Research Institution: Utsunomiya University
• Principal Investigator: XIE Xiaonan 宇都宮大学, バイオサイエンス教育研究センター, 助教 (30610323)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ストリゴラクトン / 立体化学 / 立体選択 / 構造決定 / 植物ホルモン

Outline of Final Research Achievements

Strigolactones (SLs) are carotenoid-derived signaling molecules that mediate symbiotic and parasitic communications in the rhizosphere and plant hormones that regulate the growth and development of plants through crosstalk with other hormones. Natural SLs are classified into two groups based on the stereochemistry of the B－C ring junction. In angiosperms, rice and cucumber produce orobanchol-type SLs, while cotton and strawberry produce strigol-type SLs. By contrast, Chinese milk vetch is a producer of both types of SLs. Tobacco plants also produce both types of SLs, and the ratio of orobanchol- to strigol-type SLs significantly differ between cultivars. In this study, we demonstrate that such species-specific phenomena in SL production also occur in the transport of exogenous SLs from roots to shoots.

Research Products

• [Int'l Joint Research] Nanjing Agricultural University(China)
• [Journal Article] Identification of Jasmonic Acid and Jasmonoyl-Isoleucine, and Characterization of AOS, AOC, OPR and JAR1 in the Model Lycophyte Selaginella moellendorffii. (2017)
  • Author(s): Pratiwi P, Tanaka G, Takahashi T, Xie X, Yoneyama K, Matsuura H, Takahashi K.
  • Journal Title: Plant Cell Physiol. Volume: 58 Issue: 4 Pages: 789-801
  • DOI: 10.1093/pcp/pcx031
  • NAID: 120006416010
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Complete Genome Sequence of <i>Pseudomonas chlororaphis</i> subsp. <i>aurantiaca</i> Reveals a Triplicate Quorum-Sensing Mechanism for Regulation of Phenazine Production (2017)
  • Author(s): Morohoshi T, Yamaguchi T, Xie X, Wang WZ, Takeuchi K, Someya N.
  • Journal Title: Microbes and Environments Volume: 32 Issue: 1 Pages: 47-53
  • DOI: 10.1264/jsme2.ME16162
  • NAID: 130005519590
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methyl zealactonoate, a novel germination stimulant for root parasitic weeds produced by maize. (2017)
  • Author(s): Xie X, Kisugi K, Yoneyama K, Nomura T, Akiyama K, Uchida K, Yokota T, McErlean CSP, Yoneyama K.
  • Journal Title: J. Pestic. Sci. Volume: 未定
  • NAID: 130006894846
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of Contribution of Nasunin to ORAC Value and Hyaluronidase Inhibitory Activity of Nasunin-containing Powder Obtained from Spent Eggplant Pre-pickling Solution (2017)
  • Author(s): 4)伊藤和子、阿久津智美、渡邊恒夫、謝肖男、小松渡、吉澤史昭、宇田靖
  • Journal Title: Nippon Shokuhin Kagaku Kogaku Kaishi Volume: 64 Issue: 1 Pages: 43-49
  • DOI: 10.3136/nskkk.64.43
  • NAID: 130005296336
  • ISSN: 1341-027X, 1881-6681
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural diversity of strigolactones and their distribution in the plant kingdom (2016)
  • Author(s): Xie X
  • Journal Title: Journal of Pesticide Science Volume: 41 Issue: 4 Pages: 175-180
  • DOI: 10.1584/jpestics.J16-02
  • NAID: 130005285067
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structure- and stereospecific transport of strigolactones from roots to shoots (2016)
  • Author(s): Xie X., Yoneyama K., Kisugi T., Nomura T., Akiyama K., Asami T., Yoneyama K.
  • Journal Title: Journal of Pesticide Science Volume: 41 Issue: 2 Pages: 55-58
  • DOI: 10.1584/jpestics.D16-009
  • NAID: 130005152443
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Open Access
• [Journal Article] Strigolactones are required for nitric oxide to induce root elongation in response to nitrogen- and phosphate-deficiency in rice (2016)
  • Author(s): Huwei Sun; Yang Bi; Jinyuan Tao; Shuangjie Huang; Mengmeng Hou; Ren Xue; Zhihao Lian; Pengyuan Gu; Koichi Yoneyama; Xiaonan Xie; Qirong Shen; Guohua Xu; Yali Zhang
  • Journal Title: Plant Cell and Environment Volume: 未定 Issue: 7 Pages: 1473-1484
  • DOI: 10.1111/pce.12709
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of N-acylhomoserine lactones produced by bacteria isolated from industrial cooling water systems (2016)
  • Author(s): N. Okutsu, T. Morohoshi, X. Xie, N. Kato, and T. Ikeda
  • Journal Title: Sensors Volume: 16 Issue: 1 Pages: 44-52
  • DOI: 10.3390/s16010044
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Strigolactones are transported from roots to shoots, although not through the xylem (2015)
  • Author(s): Xie X., Yoneyama K., Kisugi T., Nomura T., Akiyama K., Asami T., Yoneyama K.
  • Journal Title: Journal of Pesticide Science Volume: 40 Issue: 4 Pages: 214-216
  • DOI: 10.1584/jpestics.D15-045
  • NAID: 130005111001
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Open Access
• [Journal Article] A strigolactone signal is required for adventitious root formation in rice (2015)
  • Author(s): Huwei Sun; Yang Bi; Jinyuan Tao; Shuangjie Huang &middot; Mengmeng Hou &middot; Ren Xue &middot; Zhihao Lian; Pengyuan Gu; Koichi Yoneyama; Xiaonan Xie; Qirong Shen; Guohua Xu; Yali Zhang
  • Journal Title: Annals of Botany Volume: 155(7) Issue: 7 Pages: 1155-1162
  • DOI: 10.1093/aob/mcv052
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Difference in Striga -susceptibility is reflected in strigolactone secretion profile, but not in compatibility and host preference in arbuscular mycorrhizal symbiosis in two maize cultivars (2015)
  • Author(s): Kaori Yoneyama; Ryota Arakawa; Keiko Ishimoto; Hyun Il Kim; Takaya Kisugi; Xiaonan Xie; Takahito Nomurat; Fred Kanampiut; Takao Yokota; Tatsuhiro Ezawa; Koichi Yoneyama
  • Journal Title: New Phytologist Volume: 206(3) Issue: 3 Pages: 983-989
  • DOI: 10.1111/nph.13375
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Avenaol, a germination stimulant for root parasitic plants from Avena strigosa (2014)
  • Author(s): Hyn Il Kim, Takaya Kisugi, Pichit Khetkam, Xiaonan Xie, Kaori Yoneyama, Kenichi Uchida, Takao Yokota, Takahito Nomura, Christopher S. P. McErlean, Koichi Yoneyama
  • Journal Title: Phytochemistry Volume: 103 Pages: 85
  • DOI: 10.1016/j.phytochem.2014.03.030
  • Peer Reviewed
• [Journal Article] Low strigolactone root exudation: a novel mechanism of broomrape (Orobanche and Phelipanche spp.) (2014)
  • Author(s): Fernadndez-Aparicio M., Kisugi T., Xie X., Rubiales D., Yoneyama K.,
  • Journal Title: J. Agric. Food Chem. Volume: 62 Issue: 29 Pages: 7063-7071
  • DOI: 10.1021/jf5027235
  • Peer Reviewed
• [Journal Article] Strigolactones are involved in phosphate and nitrate deficiency-induced root development and auxin transport in rice. (2014)
  • Author(s): Sun H, Tao J., Liu S., Huang S., Chen S., Xie X., Yoneyama K., Zhang Y., Xu G.
  • Journal Title: J. Exp. Bot. Volume: 65 Issue: 22 Pages: 6735-6746
  • DOI: 10.1093/jxb/eru029
  • Peer Reviewed / Open Access
• [Journal Article] 7α- and 7β-Hydroxyorobanchyl acetate as germination stimulants for root parasitic weeds produced by cucumber (2014)
  • Author(s): Khetkam P., Xie X., Kisugi T., Kim H.I., Yoneyama K., Kisugi T., Uchida K., Yokota T., Nomura T., Yoneyama K.
  • Journal Title: Journal of Pesticide Science Volume: 39 Issue: 3 Pages: 121-126
  • DOI: 10.1584/jpestics.D14-038
  • NAID: 130004445312
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Carlactone is converted to carlactonoic acid by MAX1 in Arabidopsis and its methyl ester can directly interact with AtD14 in vitro (2014)
  • Author(s): Abe, S., Sado, A., Tanaka, K., Kisugi, T., Asami, K., Ota, S., Kim, H.I., Yoneyama, K., Xie, X., Ohnishi, T., Seto, Y., Yamaguchi, S., Akiyama, K., Yoneyama, K., Nomura, T.
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 50 Pages: 18084-18089
  • DOI: 10.1073/pnas.1410801111
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] キク科植物が生産する新奇ストリゴラクトンの探索 (2017)
  • Author(s): 松下 明真、松田 一彦、野村 崇人、米山 弘一、謝 肖男
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2017-03-17
• [Presentation] 「タルウマゴヤシにおけるmedicaol合成酵素遺伝子の同定に向けたnedicaol生合成経路の確定」 (2016)
  • Author(s): 森 愛美、謝 肖男、米山 弘一、秋山 康紀
  • Organizer: 植物化学調節学会第51回大会
  • Place of Presentation: 高知大学
  • Year and Date: 2016-10-28
• [Presentation] Are non-canonical strigolactones major players? (2016)
  • Author(s): Koichi Yoneyama, Xiaonan Xie, Kaori Yoneyama, Takahito Nomura, Kohki Akiyama, Christopher SP McErlean
  • Organizer: 22nd Intermational Conference on Plant Growth Substances
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Presentation] ミヤコグサが生産する新規ストリゴラクトンの探索 (2016)
  • Author(s): 謝 肖男、松下明真、米山香織、内田健一、横田孝雄、野村崇人、秋山康紀、米山弘一
  • Organizer: 農芸化学会2016年度大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-27
• [Presentation] 根部から地上部への構造・立体化学特異的なストリゴラクトンの輸送 (2016)
  • Author(s): 謝 肖男、米山香織、来生貴也、野村崇人、秋山康紀、浅見忠男、米山弘一
  • Organizer: 日本農薬学会第41回大会
  • Place of Presentation: 島根
  • Year and Date: 2016-03-17
• [Presentation] 根寄生植物の種子発芽刺激物質ストリゴラクトンの構造解析研究 (2016)
  • Author(s): 謝 肖男
  • Organizer: 第15回日本農学進歩賞
  • Place of Presentation: 東京大学、弥生講堂
  • Invited
• [Presentation] シダ植物イヌカタヒバが生産するストリゴラクトンの探索 (2015)
  • Author(s): 山口 慎、米山弘一、野村崇人、謝 肖男
  • Organizer: 植物化学調節学会第50回記念大会
  • Place of Presentation: 東京
  • Year and Date: 2015-10-28
• [Presentation] ストリゴール型ストリゴラクトン生産植物であるミヤゴクサ及びワタのMAX1酵素の機能解析 (2015)
  • Author(s): 森 愛美、謝 肖男、野村崇人、米山弘一、 秋山康紀
  • Organizer: 植物化学調節学会第50回記念大会
  • Place of Presentation: 東京
  • Year and Date: 2015-10-28
• [Presentation] Structure- and stereo-specific transport of strigolactones from roots to shoots. (2015)
  • Author(s): Xiaonan Xie; Kaori Yoneyama ; Takaya Kisugi; Takahito Nomura; Kohki Akiyama; Tadao Asami; Koichi Yoneyama
  • Organizer: The 13th World Congress on Parasitic Plants
  • Place of Presentation: Kunming, China
  • Year and Date: 2015-07-05
  • Int'l Joint Research / Invited
• [Presentation] ミヤコグサおよびタルウマゴヤシにおけるカーラクトンMAX1酸化産物のストリゴラクトンへの変換 (2015)
  • Author(s): 秋山康紀、佐渡愛香、徳永珠美、謝 肖男、米山弘一
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] 酵母異種発現系によるミヤコグサおよびタルウマゴヤシにMAX1酵素の機能解析 (2015)
  • Author(s): 森 愛美、謝 肖男、野村崇人、米山弘一、秋山康紀
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] 新規ストリゴラクトンzealactoneの単離および構造決定 (2015)
  • Author(s): 謝 肖男、来生貴也、米山香織、内田健一、横田孝雄、野村崇人、米山弘一、
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] ストリゴラクトンの多様な構造と植物の言葉 (2014)
  • Author(s): 謝 肖男
  • Organizer: 第二回天然物化学研究会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2014-11-23
  • Invited