Project/Area Number |
26850072
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Bioorganic chemistry
|
Research Institution | Toyama Prefectural University |
Principal Investigator |
Nomura Taiji 富山県立大学, 工学部, 准教授 (40570924)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 二次代謝 / 生合成 / 酵素 / 糖エステル / チューリッポシド / チューリッパリン / チューリッポシド変換酵素 / カルボキシルエステラーゼ |
Outline of Final Research Achievements |
This study was performed to find a clue to the biosynthesis of 6-tuliposides (6-Pos) and tulipalins (Pa), which are the major defensive secondary metabolites in tulip, and the following results were obtained: 1) 6-Acyl glucose type of Pos (6-Pos) is most probably synthesized via 1,6-diacyl type of Pos. 2) Content of 1,6-diacyl type of Pos, the putative biosynthetic intermediates for 6-Pos, differs greatly between cultivated and wild tulip species. 3) Multiple isozymes exist for Pos-converting enzyme, which catalyzes the conversion of 6-Pos to Pa, and expression profile of each isozyme gene differs with tissue, indicating that tulip subtilizes the multiple isozymes of Pos-converting enzyme depending on the tissue.
|