| Project/Area Number |
26850074
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Tohoku University (2015)
National Institute of Advanced Industrial Science and Technology (2014) |
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Principal Investigator |
ENOMOTO Masaru 東北大学, (連合)農学研究科(研究院), 准教授 (90546342)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | クラスター効果 / 天然物 / 金ナノ粒子 / 抗菌物質 / ケモセンサー / 抗菌活性 / プラジマイシン |
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| Outline of Final Research Achievements |
Multivalent interaction that enhances affinity between proteins and ligands is called a cluster effect. We evaluated the binding affinity and antifungal activity of PRM-AuNPs, which was synthesized by functionalizing gold nanoparticles (AuNPs) with pradimicin (PRM), antifungal and anti-HIV natural product. Consequently, the binding affinity of PRM-AuNPs to mannose (Man) was found to increased ten times as strong as the case of PRM although the antifungal activity of PRM-AuNPs was slightly decreased. Moreover, by making use of the distance-dependent optical properties of AuNPs as well as the Man-specific binding ability of PRM, we have developed a chemosensor that was capable of recognizing mannose residues in oligosaccharides.
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