Enhancement of health functions of bifidobacteria by polyphenols and its underlying molecular mechanisms

• Project/Area Number: 26850085
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Kobe Gakuin University (2016); Fukui Prefectural University (2014-2015)
• Principal Investigator: Kawabata Kyuichi 神戸学院大学, 栄養学部, 講師 (60452645)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ビフィズス菌 / ポリフェノール / 抗炎症作用 / 機能的相互作用 / プロテオミクス / 代謝

Outline of Final Research Achievements

We performed the proteomic analysis to investigate an action mechanism of polyphenol for enhancing the anti-inflammatory activity of bifidobacteria. Bifidobacterium adolescentis JCM1275 and1251, which are sensitive and insensitive strains to quercetin, respectively, were treated with querceitn for 3h under the anaerobic condition. Proteins extracted from these bacteria were separated by 2D electrophosis (isoelectric focusing and SDS-PAGE). Gel image analysis showed six spots with statistically significant differences, suggesting that these spots may be a protein associated with the quercetin-enhanced anti-inflammatory activity of bifidobacteria. Additionally, the binding level of quercein to the outer membrane of JCM1275 strain was 10-fold higher than that of JCM1251 strain, indicating that quercetin may affect a membrane protein of bifidobacteria to induce the anti-inflammatory activity.

Research Products

• [Journal Article] Triterpenoids isolated from Ziziphus jujuba enhance glucose uptake activity in skeletal muscle cells. (2017)
  • Author(s): Kawabata K., Kitamura K., Irie K., Naruse S., Matsuura T., Uemae T., Taira S., Ohigashi H., Murakami S., Takahashi M., Kaido Y., Kawakami B.
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 印刷中
  • NAID: 130005866046
  • Peer Reviewed
• [Journal Article] Synthesis of (2S,3R,4R)-3,4-dihydroxyarginine and its inhibitory activity against nitric oxide synthase. (2016)
  • Author(s): Masuda, Y., Maruyama, C., Kawabata, K., Hamano, Y., Doi, T.
  • Journal Title: Tetrahedron Volume: 72 Issue: 36 Pages: 5602-5611
  • DOI: 10.1016/j.tet.2016.07.050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging. (2016)
  • Author(s): Hashizaki, R., Komori, H., Kazuma, K., Konno, K., Kawabata, K., Kaneko, D., Katano H., Taira S.
  • Journal Title: International Journal of Biotechnology for Wellness Industries Volume: 5(1) Pages: 1-5
  • Peer Reviewed / Open Access
• [Journal Article] Measurement of Glucose Uptake in Cultured Cells (2015)
  • Author(s): Yamamoto N., Ueda-Wakagi M., Sato T., Kawasaki K., Sawada K., Kawabata K., Akagawa M., Ashida H.
  • Journal Title: Current Protocols in Pharmacology Volume: 12.14 Issue: 1 Pages: 1-26
  • DOI: 10.1002/0471141755.ph1214s71
  • Peer Reviewed
• [Journal Article] Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis. (2015)
  • Author(s): Kyuichi Kawabata, Yuri Kato, Taiken Sakano, Nobuyuki Baba, Kota Hagiwara, Akira Tamura, Seigo Baba, Midori Natsume, Hajime Ohigashi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 印刷中 Issue: 5 Pages: 799-807
  • DOI: 10.1080/09168451.2015.1006566
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quercetin and related polyphenols: new insights and implications for their bioactivity and bioavailability. (2015)
  • Author(s): Kyuichi Kawabata, Rie Mukai, Akari Ishisaka
  • Journal Title: Food & Function Volume: 印刷中 Issue: 5 Pages: 1399-1417
  • DOI: 10.1039/c4fo01178c
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 分子会合性を有するポリフェノールの構造特性 (2016)
  • Author(s): 豊島亮太、竹内風香、安井美菜、川畑球一、石井剛志
  • Organizer: 第21回日本フードファクター学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 2016-11-19
• [Presentation] 茶ポリフェノールの水溶液中における分子会合性 (2016)
  • Author(s): 豊島亮太、竹内風香、安井美菜、川畑球一、石井剛志
  • Organizer: 第13回日本カテキン学会年次学術集会
  • Place of Presentation: 静岡
  • Year and Date: 2016-10-27
• [Presentation] ポリフェノールによるビフィズス菌の機能性向上～カテキンの有用性～ (2016)
  • Author(s): 川畑球一
  • Organizer: 第13回日本カテキン学会年次学術大会
  • Place of Presentation: 静岡
  • Year and Date: 2016-10-27
  • Invited
• [Presentation] 非栄養性成分と腸内細菌の機能性発現における相互作用 (2016)
  • Author(s): 川畑球一
  • Organizer: 第22回FSF学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2016-09-08
  • Invited
• [Presentation] ビフィズス菌のポリフェノール応答性および機能的変化に関するプロテオーム解析 (2016)
  • Author(s): 川畑球一、萩原光太、馬場信行、村上 茂、田村 明 、馬場星吾 、夏目みどり、石井剛志
  • Organizer: 日本農芸化学会関西支部第494回講演会
  • Place of Presentation: 京都
• [Presentation] ビフィズス菌の抗炎症作用に関する特性解析 (2016)
  • Author(s): 川畑球一、馬場信行、萩原光太、村上 茂、田村 明 、夏目みどり、石井剛志
  • Organizer: 日本農芸化学会関西支部第495回講演会
  • Place of Presentation: 大阪
• [Presentation] ナツメの血糖値調節作用における食物繊維の可能性 (2016)
  • Author(s): 川畑球一
  • Organizer: 日本応用糖質科学会 第42回近畿支部会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Comparison and proteomic analysis of anti-inflammatory activity and quercetin responsivity among strains of Bifidobacterium adolescentis. (2015)
  • Author(s): Kawabata K., Hagiwara K., Baba N., Tamura A., Natsume M.
  • Organizer: 7th International Conference on Polyphenols and Health
  • Place of Presentation: Tours, France
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] Bifidobacterium adolescentisの株間における抗炎症活性およびケルセチン応答性の比較解析 (2014)
  • Author(s): 萩原光太、馬場信行、田村 明、馬場星吾、夏目みどり、川畑球一
  • Organizer: 第19回日本フードファクター学会
  • Place of Presentation: 鹿児島大学 郡元キャンパス
  • Year and Date: 2014-11-08 – 2014-11-09
• [Remarks] 神戸学院大学 栄養学部 栄養学科 食品機能学部門ホームページ
  • URL: http://www.nutr.kobegakuin.ac.jp/~foodsci/
• [Patent(Industrial Property Rights)] 有機酸の産生促進剤、並びに炎症性腸疾患の予防及び／又は改善剤 (2014)
  • Inventor(s): 田村 明、夏目みどり、川畑球一
  • Industrial Property Rights Holder: 株式会社明治
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-264242
  • Filing Date: 2014-12-26