| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We performed the proteomic analysis to investigate an action mechanism of polyphenol for enhancing the anti-inflammatory activity of bifidobacteria. Bifidobacterium adolescentis JCM1275 and1251, which are sensitive and insensitive strains to quercetin, respectively, were treated with querceitn for 3h under the anaerobic condition. Proteins extracted from these bacteria were separated by 2D electrophosis (isoelectric focusing and SDS-PAGE). Gel image analysis showed six spots with statistically significant differences, suggesting that these spots may be a protein associated with the quercetin-enhanced anti-inflammatory activity of bifidobacteria. Additionally, the binding level of quercein to the outer membrane of JCM1275 strain was 10-fold higher than that of JCM1251 strain, indicating that quercetin may affect a membrane protein of bifidobacteria to induce the anti-inflammatory activity.
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