Lineage diversification of two Cupressaceae conifer genera in New Caledonia

• Project/Area Number: 26850098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Kyoto University (2015-2016); The University of Tokyo (2014)
• Principal Investigator: Sakaguchi Shota 京都大学, 人間・環境学研究科, 助教 (50726809)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 針葉樹 / ニューカレドニア / 蛇紋岩 / 進化 / ニューカドニア / 適応進化 / 集団遺伝 / 景観遺伝 / 個体数減少 / 保全遺伝 / RNA-seq

Outline of Final Research Achievements

This project studied two conifer genera of Callitris and Libocedrus in Cupressaceae to detect the genes which would have played roles in environmental adaptation in New Caledonia. RNA-seq based phylogenetic analyses provided evidences for accelerated molecular evolution in the genes including macroelement transporter and stress-response functions. Population demographic analysis was particularly performed for one Callitris species to show complex river system and mountain barriers promoted significant population differentiation even for wind-pollinated conifers in a small geographic range.

Research Products

• [Int'l Joint Research] Agronomic Institute of New Caledonia(ニューカレドニア)
• [Journal Article] Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing (2017)
  • Author(s): Sakaguchi, S., Ueno, S., Tsumura, Y., Setoguchi, H., Ito, M., Hattori, C., Nozoe, S., Takahashi, D., Nakamasu, R., Sakagami, T., Lannuzel, G., Fogliani, B., Wulff, A.S., L’Huillier, L. and Isagi, Y.
  • Journal Title: Applications in Plant Sciences Volume: 印刷中
  • NAID: 120007129090
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Fire is a major driver of the genetic diversity of two co-occurring Tasmanian palaeo-endemic conifers (2016)
  • Author(s): James R. P. Worth, Gregory J. Jordan, James R. Marthick, Sakaguchi, S., Eric A. Colhoun, Grant J. Williamson, Motomi Ito and David J.M.S Bowman
  • Journal Title: Journal of Biogeography Volume: － Issue: 6 Pages: 1254-1267
  • DOI: 10.1111/jbi.12919
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gondwanan conifer clones imperilled by bushfire (2016)
  • Author(s): James R. P. Worth, Sakaguchi, S., Karl D. Rann, Clarence J. W. Bowman, Motomi Ito, Gregory J. Jordan, David M. J. S. Bowman
  • Journal Title: Scientific Reports Volume: － Issue: 1
  • DOI: 10.1038/srep33930
  • NAID: 120005997853
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transient hybridization, not homoploid hybrid speciation, between ancient and deeply divergent conifers (2016)
  • Author(s): Worth, J.R.P, Larcombe, M.J., Sakaguchi, S., Marthick, J.R., Bowman, D.M.J.S., Ito, M. and Jordan, G.J.
  • Journal Title: American Journal of Botany Volume: 103 Issue: 2 Pages: 246-259
  • DOI: 10.3732/ajb.1500433
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae) (2015)
  • Author(s): Sakaguchi, S., Lannuzel, G., Fogliani, B., Wulff, A. S., L’Huillier, L., Kurata, S., Ueno, S., Isagi, Y., Tsumura, Y., Ito, M.
  • Journal Title: Applications in Plant Sciences Volume: 3 Issue: 8 Pages: 1-3
  • DOI: 10.3732/apps.1500045
  • NAID: 120007136128
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] High-throughput linkage mapping of Australian white cypress pine (Callitris glaucophylla) and map transferability to related species (2015)
  • Author(s): Sakaguchi, S., Sugino, T., Tsumura, Y., Ito, M., Crisp, M.D., Bowman, D.M.J.S., Nagano, A.J., Honjo, M.N., Yasugi, M., Kudoh, H., Matsuki, Y., Suyama, Y. and Isagi, Y.
  • Journal Title: Tree Genetics & Genomes Volume: 11 Issue: 6 Pages: 1-12
  • DOI: 10.1007/s11295-015-0944-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of nuclear microsatellite markers for the Tasmanian endemic conifer Diselma archeri Hook. F. (Cupressaceae) (2014)
  • Author(s): Sakaguchi, S., Worth, J., Jordan, G., Thomas, P. and Ito, M.
  • Journal Title: Conservation Genetics Resources Volume: なし Issue: 2 Pages: 369-372
  • DOI: 10.1007/s12686-014-0368-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development of nuclear and mitochondrial microsatellite markers for the relictual conifer genus Athrotaxis (Cupressaceae) (2014)
  • Author(s): Sakaguchi, S., Worth, J., Jordan, G., Thomas, P. and Ito, M.
  • Journal Title: Conservation Genetics Resources Volume: なし Issue: 2 Pages: 477-481
  • DOI: 10.1007/s12686-014-0400-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Environmental niche modelling fails to predict Last Glacial Maximum refugia: niche shifts, microrefugia or incorrect palaeoclimate estimates? (2014)
  • Author(s): Worth, J., Williamson, G., Sakaguchi, S., Nevill, P., Jordan, G.
  • Journal Title: Global Ecology and Biogeography Volume: 23 Issue: 11 Pages: 1186-1197
  • DOI: 10.1111/geb.12239
  • Peer Reviewed
• [Journal Article] 地理情報システム技術を用いた歴史生物地理学的解析 (2014)
  • Author(s): 岩崎貴也・阪口翔太・横山良太・高見泰興・大澤剛士・池田紘士・陶山佳久
  • Journal Title: 日本生態学会誌 Volume: 64 Pages: 183-199
  • Peer Reviewed
• [Presentation] ニューカレドニア産希少針葉樹Callitris sulcataの保全遺伝解析 (2016)
  • Author(s): 阪口翔太, Guillaume Lannuzel, Bruno Fogliani, Adrien Wulff, LʼHuillier Laurent, 上野真義 , 津村義彦, 伊藤元己, 井鷺裕司
  • Organizer: 日本森林学会第127回大会
  • Place of Presentation: 神奈川県藤沢市
  • Year and Date: 2016-03-27
• [Presentation] RAD-sequencing as a tool for unravelling the population dynamics of ecologically diverse plant lineages (2015)
  • Author(s): Sakaguchi, S.
  • Organizer: Workshop of Young Scientists on Biodiversity and Conservation Biology in Southwest China: Advances in Conservation Genetics
  • Place of Presentation: 中国雲南省昆明市
  • Year and Date: 2015-07-07
  • Int'l Joint Research / Invited
• [Presentation] ニューカレドニア産ヒノキ科樹木の遺伝子における自然選択の痕跡 (2015)
  • Author(s): 阪口翔太, 上野真義, 津村義彦, 伊藤元己, James Worth, Bruno Fogliani, 井鷺裕司
  • Organizer: 第62回日本生態学会大会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2015-03-19
• [Presentation] 歴史的な景観変化が地域集団の分布・デモグラフィに及ぼす影響 (2015)
  • Author(s): 阪口翔太, David Bowman, Lynda Prior, Michael Crisp, Linde Celeste, 津村義彦, 伊藤元己, 井鷺裕司
  • Organizer: 第62回日本生態学会大会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2015-03-19
  • Invited
• [Presentation] 高密度連鎖地図上でのヒノキ科Callitris属のゲノム分化推定 (2015)
  • Author(s): 阪口翔太, 杉野壮, Michael Crisp, 永野惇, 本庄三恵, 工藤洋, 津村義彦, 伊藤元己, 井鷺裕司
  • Organizer: 日本植物分類学会第14回大会
  • Place of Presentation: 福島大学（福島県福島市）
  • Year and Date: 2015-03-07
• [Presentation] Plant demographic responses to the past environmental changes: lessons to predict the future population consequences (2015)
  • Author(s): S. Sakaguchi
  • Organizer: Contribution of Genetics to Plant Conservation, FAPESP-JSPS Joint Research Workshop 2015
  • Place of Presentation: Piracicaba, Brazil
  • Year and Date: 2015-02-03
  • Invited
• [Funded Workshop] 蛇紋岩地帯における植物多様化に迫る ―自然史・生物地理・適応― (2017)
  • Place of Presentation: 東京（早稲田大学）