| Project/Area Number |
26850103
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Forest Research and Management Organization |
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Principal Investigator |
Tsubomura Miyoko 国立研究開発法人森林研究・整備機構, 森林総合研究所林木育種センター, 主任研究員 (70415040)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | スギ / 雄性不稔 / 花粉症対策 / マーカー / 遺伝マーカー / 連鎖解析 / 花粉症 / 遺伝子 / 花粉 |
|---|
| Outline of Final Research Achievements |
The causative gene of a male-sterile Japanese cedar ‘Sosyun’ was investigated to develop a molecular marker for identifying male-sterile trees. The result of transcriptome analysis suggested that the causative gene of Sosyun has expressed at the meiosis stage or at the former stages. From the RNA sequencing data, the linkage analysis and QTL analysis, several SNP (Single Nucleotide Polymorphism)s tightly linked with the male-sterile trait were selected. We developed the SNP markers using these selected SNPs and applied them to the progeny of Sosyun. One SNP marker was able to detect not only the male-sterile individuals but also the heterozygous ones.
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