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Elucidation of toxicity mechanism(s) of Pleurocybella porrigens and creation of enzymes with novel substrate cleavage properties.

Research Project

Project/Area Number 26850110
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionUtsunomiya University (2015)
Shizuoka University (2014)

Principal Investigator

Suzuki Tomohiro  宇都宮大学, バイオサイエンス教育研究センター, 准教授 (10649601)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsきのこ / スギヒラタケ / キノコ / プロテアーゼ活性
Outline of Final Research Achievements

We have tried to obtain the gene which is interacted with PPL by a yeast two-hybrid method. As a result, the mixture (rPPL and recombinant clone protein) showed protease activity. Occasionally PPL showed the protease activity when it contained a minor peak at m/z 13,000 in MALDI-TOF-MS. We isolated PP-13,000 by reversed phase liquid chromatography and partial amino acid sequence was determined by LC-MS/MS analysis.We constructed heterologous expression systems of recombinant PPL by the basidiomycete P. sordida YK-624.We developed genomic and transcriptomic database of this mushroom which was equipped with three search functions; Keyword Search, GO Tree View and BLAST Search.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Remarks (3 results)

  • [Journal Article] A-WINGS: an integrated genome database for Pleurocybella porrigens (Angel’s wing oyster mushroom, Sugihiratake)2014

    • Author(s)
      Naoki Yamamoto, Tomohiro Suzuki, Masaaki Kobayashi, Hideo Dohra, Yohei Sasaki, Hirofumi Hirai, Koji Yokoyama, Hirokazu Kawagishi and Kentaro Yano
    • Journal Title

      BMC Research Notes

      Volume: 7 Issue: 1 Pages: 866-866

    • DOI

      10.1186/1756-0500-7-866

    • NAID

      120005575802

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Heterologous expression of a lectin from Pleurocybella porrigens (PPL) in Phanerochaete sordida YK-6242014

    • Author(s)
      T. Suzuki, H. Dohra, S. Omae, Y. Takeshima, J-H. Choi, H. Hirai, H. Kawagishi
    • Journal Title

      J. Microbiol. Meth.

      Volume: 100 Pages: 70-76

    • DOI

      10.1016/j.mimet.2014.02.016

    • NAID

      120005401892

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] スギヒラタケ由来 毒性高分子群異種発現系の構築2015

    • Author(s)
      亀澤 哲弥、竹島 愛乃、鈴木 智大 、 河岸 洋和 、平井 浩文
    • Organizer
      日本木材学会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-03-18
    • Related Report
      2014 Research-status Report
  • [Remarks] 宇都宮大学 生物分子情報学研究室

    • URL

      http://c-bio.mine.utsunomiya-u.ac.jp/suzuki/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 静岡大学大学院農学研究科応用生物化学専攻 生物化学研究室ホームページ

    • URL

      http://www.agr.shizuoka.ac.jp/c/biochem/achievement_suzuki.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 静岡大学グリーン科学技術研究所

    • URL

      http://www.green.shizuoka.ac.jp/lab_staff00028.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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