| Project/Area Number |
26850124
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Kochi University |
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Principal Investigator |
Imajoh Masayuki 高知大学, 教育研究部自然科学系農学部門, 講師 (20565741)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Nocardia seriolae / ワクチン / ドラフトゲノム / ブリ / リアルタイムPCR法 / 体内動態 / 相同組換え法 / mce遺伝子 / 相同組換え |
|---|
| Outline of Final Research Achievements |
Nocardiosis caused by Nocardia seriolae is a major disease in cultured fishes of the genus Seriola in Japan. The whole genome sequence of the virulent strain N-2927 (NBRC 110360) and the non-virulent strain U-1 (NBRC 110359) was sequenced and annotated. The draft genome sequence of N-2927 has a total of 7,758,286 bp with a G+C content of 68.3%, and that of U-1 has a total of 7,766,019 bp with a G+C content of 68.3%. Mce gene, nocovactin biosynthesis gene, and polyketide synthase gene were identified as a candidate of pathogenic factor. Bath challenge of yellowtail Seriola quinqueradiata with strain N-2927 was mimic to natural exposure. An approach by using SYBR Green real-time PCR assay may be useful for in vivo evaluating vaccination effectiveness against nocardiosis in fish. A mce gene knockout strain of N. seriolae was made by homologous recombination, but the pathogenicity to yellowtail was not attenuated.
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